DNA fusion vaccines incorporating IL-23 or RANTES for use in immunization against influenza.
The incorporation of RANTES or IL-23 into DNA vaccines may improve their immunogenicity by the recruitment and activation of dendritic cells. This may also select for a TH1 response counteracting the TH2 response which can predominate when a DNA vaccine is delivered by gene gun. We have immunized mi...
| Main Authors: | , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2008
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| _version_ | 1826280738582953984 |
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| author | Williman, J Young, S Buchan, G Slobbe, L Wilson, M Pang, P Austyn, J Preston, S Baird, M |
| author_facet | Williman, J Young, S Buchan, G Slobbe, L Wilson, M Pang, P Austyn, J Preston, S Baird, M |
| author_sort | Williman, J |
| collection | OXFORD |
| description | The incorporation of RANTES or IL-23 into DNA vaccines may improve their immunogenicity by the recruitment and activation of dendritic cells. This may also select for a TH1 response counteracting the TH2 response which can predominate when a DNA vaccine is delivered by gene gun. We have immunized mice with various DNA constructs encoding APR/8/34 influenza virus hemagglutinin (HA), either fused to or separate from, IL-23 or RANTES using a gene gun. Those immunized with IL-23/HA fusion constructs and challenged with influenza 27 weeks post-vaccination, tended to have cleared more virus than those vaccinated with HA DNA. Mice immunized with the RANTES/HA fusion construct produced a mixed TH1/TH2 response whereas in HA-vaccinated mice, a TH2 response predominated. Immunization with a plasmid in which HA and RANTES were under the control of separate promoters, failed to generate a mixed TH1/TH2 response suggesting that enhanced antigen uptake via RANTES receptors may contribute to the mixed immune response generated to the fusion construct. Overall these findings provide further evidence that Type 1 cytokines or chemokines, fused to antigen in a DNA vaccine, can influence the nature and the longevity of the immune response and ultimately, its protective capacity. |
| first_indexed | 2024-03-07T00:18:13Z |
| format | Journal article |
| id | oxford-uuid:7b97c633-18aa-4d5a-b6ff-cf5cbb3d52bd |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T00:18:13Z |
| publishDate | 2008 |
| record_format | dspace |
| spelling | oxford-uuid:7b97c633-18aa-4d5a-b6ff-cf5cbb3d52bd2022-03-26T20:51:42ZDNA fusion vaccines incorporating IL-23 or RANTES for use in immunization against influenza.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:7b97c633-18aa-4d5a-b6ff-cf5cbb3d52bdEnglishSymplectic Elements at Oxford2008Williman, JYoung, SBuchan, GSlobbe, LWilson, MPang, PAustyn, JPreston, SBaird, MThe incorporation of RANTES or IL-23 into DNA vaccines may improve their immunogenicity by the recruitment and activation of dendritic cells. This may also select for a TH1 response counteracting the TH2 response which can predominate when a DNA vaccine is delivered by gene gun. We have immunized mice with various DNA constructs encoding APR/8/34 influenza virus hemagglutinin (HA), either fused to or separate from, IL-23 or RANTES using a gene gun. Those immunized with IL-23/HA fusion constructs and challenged with influenza 27 weeks post-vaccination, tended to have cleared more virus than those vaccinated with HA DNA. Mice immunized with the RANTES/HA fusion construct produced a mixed TH1/TH2 response whereas in HA-vaccinated mice, a TH2 response predominated. Immunization with a plasmid in which HA and RANTES were under the control of separate promoters, failed to generate a mixed TH1/TH2 response suggesting that enhanced antigen uptake via RANTES receptors may contribute to the mixed immune response generated to the fusion construct. Overall these findings provide further evidence that Type 1 cytokines or chemokines, fused to antigen in a DNA vaccine, can influence the nature and the longevity of the immune response and ultimately, its protective capacity. |
| spellingShingle | Williman, J Young, S Buchan, G Slobbe, L Wilson, M Pang, P Austyn, J Preston, S Baird, M DNA fusion vaccines incorporating IL-23 or RANTES for use in immunization against influenza. |
| title | DNA fusion vaccines incorporating IL-23 or RANTES for use in immunization against influenza. |
| title_full | DNA fusion vaccines incorporating IL-23 or RANTES for use in immunization against influenza. |
| title_fullStr | DNA fusion vaccines incorporating IL-23 or RANTES for use in immunization against influenza. |
| title_full_unstemmed | DNA fusion vaccines incorporating IL-23 or RANTES for use in immunization against influenza. |
| title_short | DNA fusion vaccines incorporating IL-23 or RANTES for use in immunization against influenza. |
| title_sort | dna fusion vaccines incorporating il 23 or rantes for use in immunization against influenza |
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