Phenotypic characterization of HIV-specific CD8+ T cells during early and chronic infant HIV-1 infection.
Although CD8(+) T cells play an important role in the containment of adult HIV-1 replication, their role in infant HIV-1 infection is not as well understood. Impaired HIV-specific CD8(+) T cell responses may underlie the persistently high viral loads observed in infants. We examined the frequency an...
| Main Authors: | , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
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Public Library of Science
2011
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| _version_ | 1797078047324635136 |
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| author | Slyker, J John-Stewart, G Dong, T Lohman-Payne, B Reilly, M Atzberger, A Taylor, S Maleche-Obimbo, E Mbori-Ngacha, D Rowland-Jones, S |
| author_facet | Slyker, J John-Stewart, G Dong, T Lohman-Payne, B Reilly, M Atzberger, A Taylor, S Maleche-Obimbo, E Mbori-Ngacha, D Rowland-Jones, S |
| author_sort | Slyker, J |
| collection | OXFORD |
| description | Although CD8(+) T cells play an important role in the containment of adult HIV-1 replication, their role in infant HIV-1 infection is not as well understood. Impaired HIV-specific CD8(+) T cell responses may underlie the persistently high viral loads observed in infants. We examined the frequency and phenotype of infant HIV-specific CD8(+) T cells in 7 HIV-infected antiretroviral therapy-naive infants during the first 2 years of life, using class I HLA tetramers and IFN-(Gamma)-ELISPOT. The frequency (0.088-3.9% of CD3(+)CD8(+) cells) and phenotype (CD27(+)CD28(-), CD45RA(+/-), CD57(+/-), HLA-DR(+), CD95(+)) of infant HIV-specific CD8(+) T cells were similar to reports in adults undergoing early infection. Unlike adults, at 23-24 months post-infection a high frequency of HIV-specific CD8(+) T cells expressed HLA-DR (mean 80%, range 68-85%) and CD95 (mean 88%, range 79-96%), suggesting sustained activation and vulnerability to apoptosis. Despite comparable expansion of HIV-specific CD8(+) T cells of a similar phenotype to adults during early infection, infant T cells failed to contain HIV-1 replication, and remained persistently activated and vulnerable to apoptosis during chronic infection. |
| first_indexed | 2024-03-07T00:26:51Z |
| format | Journal article |
| id | oxford-uuid:7e6c312f-06f4-4a23-b0b0-435f8921e6be |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T00:26:51Z |
| publishDate | 2011 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | oxford-uuid:7e6c312f-06f4-4a23-b0b0-435f8921e6be2022-03-26T21:10:06ZPhenotypic characterization of HIV-specific CD8+ T cells during early and chronic infant HIV-1 infection.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:7e6c312f-06f4-4a23-b0b0-435f8921e6beEnglishSymplectic Elements at OxfordPublic Library of Science2011Slyker, JJohn-Stewart, GDong, TLohman-Payne, BReilly, MAtzberger, ATaylor, SMaleche-Obimbo, EMbori-Ngacha, DRowland-Jones, SAlthough CD8(+) T cells play an important role in the containment of adult HIV-1 replication, their role in infant HIV-1 infection is not as well understood. Impaired HIV-specific CD8(+) T cell responses may underlie the persistently high viral loads observed in infants. We examined the frequency and phenotype of infant HIV-specific CD8(+) T cells in 7 HIV-infected antiretroviral therapy-naive infants during the first 2 years of life, using class I HLA tetramers and IFN-(Gamma)-ELISPOT. The frequency (0.088-3.9% of CD3(+)CD8(+) cells) and phenotype (CD27(+)CD28(-), CD45RA(+/-), CD57(+/-), HLA-DR(+), CD95(+)) of infant HIV-specific CD8(+) T cells were similar to reports in adults undergoing early infection. Unlike adults, at 23-24 months post-infection a high frequency of HIV-specific CD8(+) T cells expressed HLA-DR (mean 80%, range 68-85%) and CD95 (mean 88%, range 79-96%), suggesting sustained activation and vulnerability to apoptosis. Despite comparable expansion of HIV-specific CD8(+) T cells of a similar phenotype to adults during early infection, infant T cells failed to contain HIV-1 replication, and remained persistently activated and vulnerable to apoptosis during chronic infection. |
| spellingShingle | Slyker, J John-Stewart, G Dong, T Lohman-Payne, B Reilly, M Atzberger, A Taylor, S Maleche-Obimbo, E Mbori-Ngacha, D Rowland-Jones, S Phenotypic characterization of HIV-specific CD8+ T cells during early and chronic infant HIV-1 infection. |
| title | Phenotypic characterization of HIV-specific CD8+ T cells during early and chronic infant HIV-1 infection. |
| title_full | Phenotypic characterization of HIV-specific CD8+ T cells during early and chronic infant HIV-1 infection. |
| title_fullStr | Phenotypic characterization of HIV-specific CD8+ T cells during early and chronic infant HIV-1 infection. |
| title_full_unstemmed | Phenotypic characterization of HIV-specific CD8+ T cells during early and chronic infant HIV-1 infection. |
| title_short | Phenotypic characterization of HIV-specific CD8+ T cells during early and chronic infant HIV-1 infection. |
| title_sort | phenotypic characterization of hiv specific cd8 t cells during early and chronic infant hiv 1 infection |
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