A general method for preparing intact nuclear DNA.
Naked nuclear DNA is easily sheared. Two general methods are described for preparing intact DNA in a stable form that can be pipetted without breaking it. Cells are encapsulated in agarose microbeads and then lysed in a non-ionic detergent (i.e., Triton X-100) and 2 M NaCl or an ionic detergent (e.g...
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| Format: | Journal article |
| Language: | English |
| Published: |
1984
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