Single-molecule identification of Coumarin-120 by time-resolved fluorescence detection: Comparison of one- and two-photon excitation in solution

Using two-photon excitation (TPE) at 700 nm as well as one-photon excitation (OPE) at 350 nm, we applied confocal fluorescence microscopy to detect single Coumarin-120 molecules in the solvents water and triacetin. To study the behavior of Coumarin-120 under different excitation conditions, fluoresc...

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Bibliographic Details
Main Authors: Brand, L, Eggeling, C, Zander, C, Drexhage, K, Seidel, C
Format: Journal article
Published: 1997