Robust phosphoproteome enrichment using monodisperse microsphere-based immobilized titanium (IV) ion affinity chromatography.
Mass spectrometry (MS)-based proteomics has become the preferred tool for the analysis of protein phosphorylation. To be successful at such an endeavor, there is a requirement for an efficient enrichment of phosphopeptides. This is necessary because of the substoichiometric nature of phosphorylation...
Glavni autori: | Zhou, H, Ye, M, Dong, J, Corradini, E, Cristobal, A, Heck, A, Zou, H, Mohammed, S |
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Format: | Journal article |
Jezik: | English |
Izdano: |
2013
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Slični predmeti
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Phosphoproteomics.
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Characterization of biases in phosphopeptide enrichment by Ti(4+)-immobilized metal affinity chromatography and TiO2 using a massive synthetic library and human cell digests.
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Recovery of cyclodextrin glucanotransferase (CGTase) using immobilized metal chelating affinity chromatography
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Affinity chromatography/
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