Toll-like receptor responses in IRAK-4-deficient neutrophils.
Human neutrophils were found to express all known Toll-like receptors (TLRs) except TLR3 and TLR7. IRAK-4-deficient neutrophils were tested for their responsiveness to various TLR ligands. Essentially all TLR responses in neutrophils, including the induction of reactive oxygen species generation, ad...
| Main Authors: | , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2010
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| _version_ | 1797078394270121984 |
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| author | van Bruggen, R Drewniak, A Tool, A Jansen, M van Houdt, M Geissler, J van den Berg, T Chapel, H Kuijpers, T |
| author_facet | van Bruggen, R Drewniak, A Tool, A Jansen, M van Houdt, M Geissler, J van den Berg, T Chapel, H Kuijpers, T |
| author_sort | van Bruggen, R |
| collection | OXFORD |
| description | Human neutrophils were found to express all known Toll-like receptors (TLRs) except TLR3 and TLR7. IRAK-4-deficient neutrophils were tested for their responsiveness to various TLR ligands. Essentially all TLR responses in neutrophils, including the induction of reactive oxygen species generation, adhesion, chemotaxis and IL-8 secretion, were found to be dependent on IRAK-4. Surprisingly, the reactivity towards certain established TLR ligands, imiquimod and ODN-CpG, was unaffected by IRAK-4 deficiency, demonstrating their activity is independent of TLR. TLR-4-dependent signaling in neutrophils was totally dependent on IRAK-4 without any major TRIF-mediated contribution. We did not observe any defects in killing capacity of IRAK-4-deficient neutrophils for Staphylococcus aureus, Escherichia coli and Candida albicans, suggesting that microbial killing is primarily TLR independent. |
| first_indexed | 2024-03-07T00:31:21Z |
| format | Journal article |
| id | oxford-uuid:7fe79dd8-2080-4d16-8bac-1525fd83e986 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T00:31:21Z |
| publishDate | 2010 |
| record_format | dspace |
| spelling | oxford-uuid:7fe79dd8-2080-4d16-8bac-1525fd83e9862022-03-26T21:19:53ZToll-like receptor responses in IRAK-4-deficient neutrophils.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:7fe79dd8-2080-4d16-8bac-1525fd83e986EnglishSymplectic Elements at Oxford2010van Bruggen, RDrewniak, ATool, AJansen, Mvan Houdt, MGeissler, Jvan den Berg, TChapel, HKuijpers, THuman neutrophils were found to express all known Toll-like receptors (TLRs) except TLR3 and TLR7. IRAK-4-deficient neutrophils were tested for their responsiveness to various TLR ligands. Essentially all TLR responses in neutrophils, including the induction of reactive oxygen species generation, adhesion, chemotaxis and IL-8 secretion, were found to be dependent on IRAK-4. Surprisingly, the reactivity towards certain established TLR ligands, imiquimod and ODN-CpG, was unaffected by IRAK-4 deficiency, demonstrating their activity is independent of TLR. TLR-4-dependent signaling in neutrophils was totally dependent on IRAK-4 without any major TRIF-mediated contribution. We did not observe any defects in killing capacity of IRAK-4-deficient neutrophils for Staphylococcus aureus, Escherichia coli and Candida albicans, suggesting that microbial killing is primarily TLR independent. |
| spellingShingle | van Bruggen, R Drewniak, A Tool, A Jansen, M van Houdt, M Geissler, J van den Berg, T Chapel, H Kuijpers, T Toll-like receptor responses in IRAK-4-deficient neutrophils. |
| title | Toll-like receptor responses in IRAK-4-deficient neutrophils. |
| title_full | Toll-like receptor responses in IRAK-4-deficient neutrophils. |
| title_fullStr | Toll-like receptor responses in IRAK-4-deficient neutrophils. |
| title_full_unstemmed | Toll-like receptor responses in IRAK-4-deficient neutrophils. |
| title_short | Toll-like receptor responses in IRAK-4-deficient neutrophils. |
| title_sort | toll like receptor responses in irak 4 deficient neutrophils |
| work_keys_str_mv | AT vanbruggenr tolllikereceptorresponsesinirak4deficientneutrophils AT drewniaka tolllikereceptorresponsesinirak4deficientneutrophils AT toola tolllikereceptorresponsesinirak4deficientneutrophils AT jansenm tolllikereceptorresponsesinirak4deficientneutrophils AT vanhoudtm tolllikereceptorresponsesinirak4deficientneutrophils AT geisslerj tolllikereceptorresponsesinirak4deficientneutrophils AT vandenbergt tolllikereceptorresponsesinirak4deficientneutrophils AT chapelh tolllikereceptorresponsesinirak4deficientneutrophils AT kuijperst tolllikereceptorresponsesinirak4deficientneutrophils |