Targeted sequencing for gene discovery and quantification using RNA CaptureSeq.

RNA sequencing (RNAseq) samples the majority of expressed genes infrequently, owing to the large size, complex splicing and wide dynamic range of eukaryotic transcriptomes. This results in sparse sequencing coverage that can hinder robust isoform assembly and quantification. RNA capture sequencing (...

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Bibliographic Details
Main Authors: Mercer, T, Clark, M, Crawford, J, Brunck, M, Gerhardt, D, Taft, R, Nielsen, L, Dinger, M, Mattick, J
Format: Journal article
Language:English
Published: Nature Publishing Group 2014