Targeted sequencing for gene discovery and quantification using RNA CaptureSeq.

RNA sequencing (RNAseq) samples the majority of expressed genes infrequently, owing to the large size, complex splicing and wide dynamic range of eukaryotic transcriptomes. This results in sparse sequencing coverage that can hinder robust isoform assembly and quantification. RNA capture sequencing (...

Descripción completa

Detalles Bibliográficos
Autores principales: Mercer, T, Clark, M, Crawford, J, Brunck, M, Gerhardt, D, Taft, R, Nielsen, L, Dinger, M, Mattick, J
Formato: Journal article
Lenguaje:English
Publicado: Nature Publishing Group 2014