Functional characterisation of cis-regulatory elements governing dynamic Eomes expression in the early mouse embryo
The T-box transcription factor (TF) Eomes is a key regulator of cell fate decisions during early mouse development. The cis-acting regulatory elements that direct expression in the anterior visceral endoderm (AVE), primitive streak (PS) and definitive endoderm (DE) have yet to be defined. Here, we i...
| Principais autores: | , , , , , , , , |
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| Formato: | Journal article |
| Idioma: | English |
| Publicado em: |
Company of Biologists
2017
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| _version_ | 1826281956984225792 |
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| author | Simon, C Downes, D Gosden, M Telenius, J Higgs, D Hughes, J Costello, I Bikoff, E Robertson, E |
| author_facet | Simon, C Downes, D Gosden, M Telenius, J Higgs, D Hughes, J Costello, I Bikoff, E Robertson, E |
| author_sort | Simon, C |
| collection | OXFORD |
| description | The T-box transcription factor (TF) Eomes is a key regulator of cell fate decisions during early mouse development. The cis-acting regulatory elements that direct expression in the anterior visceral endoderm (AVE), primitive streak (PS) and definitive endoderm (DE) have yet to be defined. Here, we identified three gene-proximal enhancer-like sequences (PSE_a, PSE_b and VPE) that faithfully activate tissue specific expression in transgenic embryos. However, targeted deletion experiments demonstrate that PSE_a and PSE_b are dispensable and only the VPE is required for optimal Eomes expression in vivo Embryos lacking this enhancer display variably penetrant defects in anterior-posterior axis orientation and DE formation. Chromosome conformation capture experiments reveal VPE-promoter interactions embryonic stem cells (ESC), prior to gene activation. The locus resides in a large (500kb) pre-formed compartment in ESC and activation during DE differentiation occurs in the absence of 3D structural changes. ATAC-seq analysis reveals that VPE, PSE_a, and four additional putative enhancers display increased chromatin accessibility in DE associated with Smad2/3 binding coincident with transcriptional activation. In contrast, activation of the Eomes target genes Foxa2 and Lhx1 is associated with higher order chromatin reorganisation. Thus diverse regulatory mechanisms govern activation of lineage specifying TFs during early development. |
| first_indexed | 2024-03-07T00:36:37Z |
| format | Journal article |
| id | oxford-uuid:81a3795b-461d-4525-9376-8e9890b7b161 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T00:36:37Z |
| publishDate | 2017 |
| publisher | Company of Biologists |
| record_format | dspace |
| spelling | oxford-uuid:81a3795b-461d-4525-9376-8e9890b7b1612022-03-26T21:31:39ZFunctional characterisation of cis-regulatory elements governing dynamic Eomes expression in the early mouse embryoJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:81a3795b-461d-4525-9376-8e9890b7b161EnglishSymplectic Elements at OxfordCompany of Biologists2017Simon, CDownes, DGosden, MTelenius, JHiggs, DHughes, JCostello, IBikoff, ERobertson, EThe T-box transcription factor (TF) Eomes is a key regulator of cell fate decisions during early mouse development. The cis-acting regulatory elements that direct expression in the anterior visceral endoderm (AVE), primitive streak (PS) and definitive endoderm (DE) have yet to be defined. Here, we identified three gene-proximal enhancer-like sequences (PSE_a, PSE_b and VPE) that faithfully activate tissue specific expression in transgenic embryos. However, targeted deletion experiments demonstrate that PSE_a and PSE_b are dispensable and only the VPE is required for optimal Eomes expression in vivo Embryos lacking this enhancer display variably penetrant defects in anterior-posterior axis orientation and DE formation. Chromosome conformation capture experiments reveal VPE-promoter interactions embryonic stem cells (ESC), prior to gene activation. The locus resides in a large (500kb) pre-formed compartment in ESC and activation during DE differentiation occurs in the absence of 3D structural changes. ATAC-seq analysis reveals that VPE, PSE_a, and four additional putative enhancers display increased chromatin accessibility in DE associated with Smad2/3 binding coincident with transcriptional activation. In contrast, activation of the Eomes target genes Foxa2 and Lhx1 is associated with higher order chromatin reorganisation. Thus diverse regulatory mechanisms govern activation of lineage specifying TFs during early development. |
| spellingShingle | Simon, C Downes, D Gosden, M Telenius, J Higgs, D Hughes, J Costello, I Bikoff, E Robertson, E Functional characterisation of cis-regulatory elements governing dynamic Eomes expression in the early mouse embryo |
| title | Functional characterisation of cis-regulatory elements governing dynamic Eomes expression in the early mouse embryo |
| title_full | Functional characterisation of cis-regulatory elements governing dynamic Eomes expression in the early mouse embryo |
| title_fullStr | Functional characterisation of cis-regulatory elements governing dynamic Eomes expression in the early mouse embryo |
| title_full_unstemmed | Functional characterisation of cis-regulatory elements governing dynamic Eomes expression in the early mouse embryo |
| title_short | Functional characterisation of cis-regulatory elements governing dynamic Eomes expression in the early mouse embryo |
| title_sort | functional characterisation of cis regulatory elements governing dynamic eomes expression in the early mouse embryo |
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