Influence of neutral protease activity on human islet isolation outcome.

Influence of neutral protease activity on human islet isolation outcome.

Observations in rat pancreata have revealed that enzymatic islet release is mediated by both collagenase and neutral protease (NP), a critical effector of islet integrity. Since no information is available about the effect of NP activity on islet release from the human pancreas, the present study ev...

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Bibliografiske detaljer
Main Authors:	Brandhorst, H, Brendel, MD, Eckhard, M, Bretzel, R, Brandhorst, D
Format:	Journal article
Sprog:	English
Udgivet:	2005

Lignende værker

Adaption of neutral protease activity for islet isolation from the long-term two-layer method-stored pig pancreas.
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Udgivet: (2005)

Can the density of native pancreatic tissue slices predict human islet isolation and purification outcome?
af: Eckhard, M, et al.
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Optimization in osmolality and range of density of a continuous ficoll-sodium-diatrizoate gradient for isopycnic purification of isolated human islets.
af: Eckhard, M, et al.
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Comparison of Clostripain and Neutral Protease as Supplementary Enzymes for Human Islet Isolation
af: Brandhorst, H, et al.
Udgivet: (2018)

Quantifying the effects of different neutral proteases on human islet integrity
af: Brandhorst, H, et al.
Udgivet: (2018)

Comparison of neutral proteases and collagenase class I as essential enzymes for human islet isolation
af: Brandhorst, H, et al.
Udgivet: (2015)

Successful human islet isolation utilizing recombinant collagenase.
af: Brandhorst, H, et al.
Udgivet: (2003)

Influence of collagenase loading on long-term preservation of pig pancreas by the two-layer method for subsequent islet isolation.
af: Brandhorst, D, et al.
Udgivet: (2005)

Long-term preservation of the pig pancreas by a one-layer method for successful islet isolation.
af: Brandhorst, D, et al.
Udgivet: (2005)

Successful pancreas preservation by a perfluorocarbon-based one-layer method for subsequent pig islet isolation.
af: Brandhorst, D, et al.
Udgivet: (2005)

Pretreatment of isolated islets with caspase-3 inhibitor DEVD increases graft survival after xenotransplantation.
af: Brandhorst, H, et al.
Udgivet: (2003)

The ratio between class II and class I collagenase determines the amount of neutral protease activity required for efficient islet release from the rat pancreas.
af: Brandhorst, H, et al.
Udgivet: (2005)

The role of current product release criteria for identification of human islet preparations suitable for clinical transplantation.
af: Eckhard, M, et al.
Udgivet: (2004)

Adjustment of the ratio between collagenase class II and I improves islet isolation outcome.
af: Brandhorst, D, et al.
Udgivet: (2005)

Islet transplantation: present clinical situation and future aspects.
af: Bretzel, R, et al.
Udgivet: (2001)

Heat-exposed pig islets are protected from inflammatory mediators but express proapoptotic proteins.
af: Brandhorst, D, et al.
Udgivet: (2003)

Experience with human islet transplantation in Sweden.
af: Tibell, A, et al.
Udgivet: (2001)

Pretransplant induction of HSP-70 in isolated adult pig islets decreases early islet xenograft survival.
af: Brandhorst, D, et al.
Udgivet: (2000)

Pancreatic islets: methods for isolation and purification of juvenile and adult pig islets
af: Brandhorst, H, et al.
Udgivet: (2016)

Pancreas storage in oxygenated perfluorodecalin does not restore post-transplant function of isolated pig islets pre-damaged by warm ischemia.
af: Brandhorst, D, et al.
Udgivet: (2006)

Enzyme development for human islet isolation: Five decades of progress or stagnation?
af: Brandhorst, D, et al.
Udgivet: (2017)

Hyperthermic preconditioning protects pig islet grafts from early inflammation but enhances rejection in immunocompetent mice.
af: Brandhorst, D, et al.
Udgivet: (2003)

Vascular endothelial growth factor increases functional beta-cell mass by improvement of angiogenesis of isolated human and murine pancreatic islets.
af: Lai, Y, et al.
Udgivet: (2005)

Islet cell transplantation - Current state and perspectives
af: Brendel, MD, et al.
Udgivet: (2003)

Induction and amelioration of environmental stress in isolated islets until transplantation
af: Brandhorst, D, et al.
Udgivet: (2006)

Effect of stable glutamine compounds on porcine islet culture.
af: Brandhorst, H, et al.
Udgivet: (2005)

Prevention of early islet graft failure by selective inducible nitric oxide synthase inhibitors after pig to nude rat intraportal islet transplantation.
af: Brandhorst, D, et al.
Udgivet: (2001)

Timing of hyperthermic preconditioning affects islet resistance against inflammation.
af: Brandhorst, D, et al.
Udgivet: (2005)

Expression of complement regulatory proteins on islets of Langerhans: a comparison between human islets and islets isolated from normal and hDAF transgenic pigs.
af: Bennet, W, et al.
Udgivet: (2001)

High seeding density induces local hypoxia and triggers a proinflammatory response in isolated human islets
af: Brandhorst, D, et al.
Udgivet: (2016)

Adiponectin is functionally active in human islets but does not affect insulin secretory function or beta-cell lipoapoptosis.
af: Staiger, K, et al.
Udgivet: (2005)

Effect of etanercept concentration on human islet integrity
af: Acreman, S, et al.
Udgivet: (2017)

Ischaemia is linked to inflammation and induction of angiogenesis in pancreatic islets.
af: Linn, T, et al.
Udgivet: (2006)

High-dosed nicotinamide decreases early graft failure after pig to nude rat intraportal islet transplantation.
af: Brandhorst, D, et al.
Udgivet: (2002)

Prevention of intraportal islet failure by a highly selective iNOS inhibitor in the pig-to-nude rat model.
af: Brandhorst, D, et al.
Udgivet: (2001)

Porcine islet graft function is affected by pretreatment with a caspase-3 inhibitor.
af: Brandhorst, D, et al.
Udgivet: (2006)

Anti-caspase-3 preconditioning increases proinsulin secretion and deteriorates posttransplant function of isolated human islets.
af: Brandhorst, D, et al.
Udgivet: (2013)

Anti-caspase-3 preconditioning increases proinsulin secretion and deteriorates posttransplant function of isolated human islets
af: Brandhorst, D, et al.
Udgivet: (2013)

Basement membrane proteins improve human islet survival in hypoxia: Implications for islet inflammation
af: Brandhorst, D, et al.
Udgivet: (2021)

The effect of truncated collagenase class I isomers on human islet isolation outcome.
af: Brandhorst, H, et al.
Udgivet: (2010)