Influence of neutral protease activity on human islet isolation outcome.

Influence of neutral protease activity on human islet isolation outcome.

Observations in rat pancreata have revealed that enzymatic islet release is mediated by both collagenase and neutral protease (NP), a critical effector of islet integrity. Since no information is available about the effect of NP activity on islet release from the human pancreas, the present study ev...

Ausführliche Beschreibung

Bibliographische Detailangaben
Hauptverfasser:	Brandhorst, H, Brendel, MD, Eckhard, M, Bretzel, R, Brandhorst, D
Format:	Journal article
Sprache:	English
Veröffentlicht:	2005

Ähnliche Einträge

Adaption of neutral protease activity for islet isolation from the long-term two-layer method-stored pig pancreas.
von: Brandhorst, D, et al.
Veröffentlicht: (2005)

Can the density of native pancreatic tissue slices predict human islet isolation and purification outcome?
von: Eckhard, M, et al.
Veröffentlicht: (2004)

Optimization in osmolality and range of density of a continuous ficoll-sodium-diatrizoate gradient for isopycnic purification of isolated human islets.
von: Eckhard, M, et al.
Veröffentlicht: (2004)

Comparison of Clostripain and Neutral Protease as Supplementary Enzymes for Human Islet Isolation
von: Brandhorst, H, et al.
Veröffentlicht: (2018)

Quantifying the effects of different neutral proteases on human islet integrity
von: Brandhorst, H, et al.
Veröffentlicht: (2018)

Comparison of Clostripain and Neutral Protease as Supplementary Enzymes for Human Islet Isolation
von: Heide Brandhorst, et al.
Veröffentlicht: (2019-02-01)

Comparison of neutral proteases and collagenase class I as essential enzymes for human islet isolation
von: Brandhorst, H, et al.
Veröffentlicht: (2015)

Successful human islet isolation utilizing recombinant collagenase.
von: Brandhorst, H, et al.
Veröffentlicht: (2003)

Long-term preservation of the pig pancreas by a one-layer method for successful islet isolation.
von: Brandhorst, D, et al.
Veröffentlicht: (2005)

Quantifying the Effects of Different Neutral Proteases on Human Islet Integrity
von: Heide Brandhorst, et al.
Veröffentlicht: (2017-11-01)

Influence of collagenase loading on long-term preservation of pig pancreas by the two-layer method for subsequent islet isolation.
von: Brandhorst, D, et al.
Veröffentlicht: (2005)

Successful pancreas preservation by a perfluorocarbon-based one-layer method for subsequent pig islet isolation.
von: Brandhorst, D, et al.
Veröffentlicht: (2005)

Pretreatment of isolated islets with caspase-3 inhibitor DEVD increases graft survival after xenotransplantation.
von: Brandhorst, H, et al.
Veröffentlicht: (2003)

Assessment of Intracellular Insulin Content during All Steps of Human Islet Isolation Procedure
von: Heide Brandhorst, et al.
Veröffentlicht: (1998-09-01)

The ratio between class II and class I collagenase determines the amount of neutral protease activity required for efficient islet release from the rat pancreas.
von: Brandhorst, H, et al.
Veröffentlicht: (2005)

The role of current product release criteria for identification of human islet preparations suitable for clinical transplantation.
von: Eckhard, M, et al.
Veröffentlicht: (2004)

Heat-exposed pig islets are protected from inflammatory mediators but express proapoptotic proteins.
von: Brandhorst, D, et al.
Veröffentlicht: (2003)

Adjustment of the ratio between collagenase class II and I improves islet isolation outcome.
von: Brandhorst, D, et al.
Veröffentlicht: (2005)

Pancreas storage in oxygenated perfluorodecalin does not restore post-transplant function of isolated pig islets pre-damaged by warm ischemia.
von: Brandhorst, D, et al.
Veröffentlicht: (2006)

Pretransplant induction of HSP-70 in isolated adult pig islets decreases early islet xenograft survival.
von: Brandhorst, D, et al.
Veröffentlicht: (2000)

Hyperthermic preconditioning protects pig islet grafts from early inflammation but enhances rejection in immunocompetent mice.
von: Brandhorst, D, et al.
Veröffentlicht: (2003)

Islet transplantation: present clinical situation and future aspects.
von: Bretzel, R, et al.
Veröffentlicht: (2001)

Pancreatic islets: methods for isolation and purification of juvenile and adult pig islets
von: Brandhorst, H, et al.
Veröffentlicht: (2016)

Vascular endothelial growth factor increases functional beta-cell mass by improvement of angiogenesis of isolated human and murine pancreatic islets.
von: Lai, Y, et al.
Veröffentlicht: (2005)

Effect of stable glutamine compounds on porcine islet culture.
von: Brandhorst, H, et al.
Veröffentlicht: (2005)

Prevention of early islet graft failure by selective inducible nitric oxide synthase inhibitors after pig to nude rat intraportal islet transplantation.
von: Brandhorst, D, et al.
Veröffentlicht: (2001)

Enzyme development for human islet isolation: Five decades of progress or stagnation?
von: Brandhorst, D, et al.
Veröffentlicht: (2017)

Experience with human islet transplantation in Sweden.
von: Tibell, A, et al.
Veröffentlicht: (2001)

Timing of hyperthermic preconditioning affects islet resistance against inflammation.
von: Brandhorst, D, et al.
Veröffentlicht: (2005)

Islet cell transplantation - Current state and perspectives
von: Brendel, MD, et al.
Veröffentlicht: (2003)

Induction and amelioration of environmental stress in isolated islets until transplantation
von: Brandhorst, D, et al.
Veröffentlicht: (2006)

Pretransplant Induction of HSP-70 in Isolated Adult Pig Islets Decreases Early Islet Xenograft Survival
von: Daniel Brandhorst Ph.D., et al.
Veröffentlicht: (2000-05-01)

High-dosed nicotinamide decreases early graft failure after pig to nude rat intraportal islet transplantation.
von: Brandhorst, D, et al.
Veröffentlicht: (2002)

Prevention of intraportal islet failure by a highly selective iNOS inhibitor in the pig-to-nude rat model.
von: Brandhorst, D, et al.
Veröffentlicht: (2001)

Porcine islet graft function is affected by pretreatment with a caspase-3 inhibitor.
von: Brandhorst, D, et al.
Veröffentlicht: (2006)

Ischaemia is linked to inflammation and induction of angiogenesis in pancreatic islets.
von: Linn, T, et al.
Veröffentlicht: (2006)

Hyperthermic Preconditioning Protects Pig Islet Grafts from Early Inflammation but Enhances Rejection in Immunocompetent Mice
von: Daniel Brandhorst Ph.D., et al.
Veröffentlicht: (2003-11-01)

High seeding density induces local hypoxia and triggers a proinflammatory response in isolated human islets
von: Brandhorst, D, et al.
Veröffentlicht: (2016)

Effect of etanercept concentration on human islet integrity
von: Acreman, S, et al.
Veröffentlicht: (2017)

Basement membrane proteins improve human islet survival in hypoxia: Implications for islet inflammation
von: Brandhorst, D, et al.
Veröffentlicht: (2021)