Identification of viable and non-viable haemophilus influenzae using qPCR
Conventional real-time PCR for the detection of bacteria is unable to distinguish between viable and non-viable cells within a sample. Propidium monoazide (PMA) is a photo-reactive dye that is cell membrane impermeable which interacts with non-viable cells ceasing amplification due to DNA modificat...
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European Respiratory Society
2016
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