Identification of viable and non-viable haemophilus influenzae using qPCR
Conventional real-time PCR for the detection of bacteria is unable to distinguish between viable and non-viable cells within a sample. Propidium monoazide (PMA) is a photo-reactive dye that is cell membrane impermeable which interacts with non-viable cells ceasing amplification due to DNA modificat...
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| Format: | Conference item |
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European Respiratory Society
2016
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| _version_ | 1826283710707662848 |
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| author | Thulborn, S Bafadhel, M |
| author_facet | Thulborn, S Bafadhel, M |
| author_sort | Thulborn, S |
| collection | OXFORD |
| description | Conventional real-time PCR for the detection of bacteria is unable to distinguish between viable and non-viable cells within a sample. Propidium monoazide (PMA) is a photo-reactive dye that is cell membrane impermeable which interacts with non-viable cells ceasing amplification due to DNA modification. We investigated if PMA could distinguish between viable and non-viable Haemophilus influenzae. |
| first_indexed | 2024-03-07T01:03:00Z |
| format | Conference item |
| id | oxford-uuid:8a5bcc98-4486-44de-a218-478767cf417e |
| institution | University of Oxford |
| last_indexed | 2024-03-07T01:03:00Z |
| publishDate | 2016 |
| publisher | European Respiratory Society |
| record_format | dspace |
| spelling | oxford-uuid:8a5bcc98-4486-44de-a218-478767cf417e2022-03-26T22:30:58ZIdentification of viable and non-viable haemophilus influenzae using qPCRConference itemhttp://purl.org/coar/resource_type/c_5794uuid:8a5bcc98-4486-44de-a218-478767cf417eSymplectic Elements at OxfordEuropean Respiratory Society2016Thulborn, SBafadhel, M Conventional real-time PCR for the detection of bacteria is unable to distinguish between viable and non-viable cells within a sample. Propidium monoazide (PMA) is a photo-reactive dye that is cell membrane impermeable which interacts with non-viable cells ceasing amplification due to DNA modification. We investigated if PMA could distinguish between viable and non-viable Haemophilus influenzae. |
| spellingShingle | Thulborn, S Bafadhel, M Identification of viable and non-viable haemophilus influenzae using qPCR |
| title | Identification of viable and non-viable haemophilus influenzae using qPCR |
| title_full | Identification of viable and non-viable haemophilus influenzae using qPCR |
| title_fullStr | Identification of viable and non-viable haemophilus influenzae using qPCR |
| title_full_unstemmed | Identification of viable and non-viable haemophilus influenzae using qPCR |
| title_short | Identification of viable and non-viable haemophilus influenzae using qPCR |
| title_sort | identification of viable and non viable haemophilus influenzae using qpcr |
| work_keys_str_mv | AT thulborns identificationofviableandnonviablehaemophilusinfluenzaeusingqpcr AT bafadhelm identificationofviableandnonviablehaemophilusinfluenzaeusingqpcr |