Recombinant protein expression and solubility screening in Escherichia coli: a comparative study.

Producing soluble proteins in Escherichia coli is still a major bottleneck for structural proteomics. Therefore, screening for soluble expression on a small scale is an attractive way of identifying constructs that are likely to be amenable to structural analysis. A variety of expression-screening m...

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Bibliographic Details
Main Authors: Berrow, N, Büssow, K, Coutard, B, Diprose, J, Ekberg, M, Folkers, G, Levy, N, Lieu, V, Owens, R, Peleg, Y, Pinaglia, C, Quevillon-Cheruel, S, Salim, L, Scheich, C, Vincentelli, R, Busso, D
Format: Journal article
Language:English
Published: 2006