Highly efficient targeted mutagenesis of Drosophila with the CRISPR/Cas9 system

Here, we present a simple and highly efficient method for generating and detecting mutations of any gene in Drosophila melanogaster through the use of the CRISPR/Cas9 system (clustered regularly interspaced palindromic repeats/CRISPR-associated). We show that injection of RNA into the Drosophila emb...

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Bibliographic Details
Main Authors: Bassett, AR, Tibbit, C, Ponting, CP, Liu, J-L
Format: Journal article
Language:English
Published: Cell Press 2013