Expression and characterisation of recombinant oligomeric envelope glycoproteins derived from primary isolates of HIV-1.

The production, purification and characterisation of recombinant gp140 oligomeric envelope glycoproteins derived from six primary isolates of HIV-1 (covering clades A, B, C, D, F and O) are described. Using a Chinese hamster ovary cell expression system, expression levels of between 0.1 and 1 mg/l c...

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Bibliographic Details
Main Authors: Jeffs, SA, Goriup, S, Kebble, B, Crane, D, Bolgiano, B, Sattentau, Q, Jones, S, Holmes, H
Format: Journal article
Language:English
Published: 2004