Single-molecule analysis of G protein-coupled receptor stoichiometry: Approaches and limitations
How G protein-coupled receptors (GPCRs) are organized at the cell surface remains highly contentious. Single-molecule (SM) imaging is starting to inform this debate as receptor behavior can now be visualized directly, without the need for interpreting ensemble data. The limited number of SM studies...
| Main Authors: | , , |
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| Formato: | Journal article |
| Idioma: | English |
| Publicado em: |
Elsevier
2017
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| _version_ | 1826284329116893184 |
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| author | Felce, J Davis, S Klenerman, D |
| author_facet | Felce, J Davis, S Klenerman, D |
| author_sort | Felce, J |
| collection | OXFORD |
| description | How G protein-coupled receptors (GPCRs) are organized at the cell surface remains highly contentious. Single-molecule (SM) imaging is starting to inform this debate as receptor behavior can now be visualized directly, without the need for interpreting ensemble data. The limited number of SM studies of GPCRs undertaken to date have strongly suggested that dimerization is at most transient, and that most receptors are monomeric at any given time. However, even SM data has its caveats and needs to be interpreted carefully. Here, we discuss the types of SM imaging strategies used to examine GPCR stoichiometry and consider some of these caveats. We also emphasize that attempts to resolve the debate ought to rely on orthogonal approaches to measuring receptor stoichiometry. |
| first_indexed | 2024-03-07T01:12:13Z |
| format | Journal article |
| id | oxford-uuid:8d689fa4-c62e-450c-a310-213d0c96c8af |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T01:12:13Z |
| publishDate | 2017 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | oxford-uuid:8d689fa4-c62e-450c-a310-213d0c96c8af2022-03-26T22:51:03ZSingle-molecule analysis of G protein-coupled receptor stoichiometry: Approaches and limitationsJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:8d689fa4-c62e-450c-a310-213d0c96c8afEnglishSymplectic Elements at OxfordElsevier2017Felce, JDavis, SKlenerman, DHow G protein-coupled receptors (GPCRs) are organized at the cell surface remains highly contentious. Single-molecule (SM) imaging is starting to inform this debate as receptor behavior can now be visualized directly, without the need for interpreting ensemble data. The limited number of SM studies of GPCRs undertaken to date have strongly suggested that dimerization is at most transient, and that most receptors are monomeric at any given time. However, even SM data has its caveats and needs to be interpreted carefully. Here, we discuss the types of SM imaging strategies used to examine GPCR stoichiometry and consider some of these caveats. We also emphasize that attempts to resolve the debate ought to rely on orthogonal approaches to measuring receptor stoichiometry. |
| spellingShingle | Felce, J Davis, S Klenerman, D Single-molecule analysis of G protein-coupled receptor stoichiometry: Approaches and limitations |
| title | Single-molecule analysis of G protein-coupled receptor stoichiometry: Approaches and limitations |
| title_full | Single-molecule analysis of G protein-coupled receptor stoichiometry: Approaches and limitations |
| title_fullStr | Single-molecule analysis of G protein-coupled receptor stoichiometry: Approaches and limitations |
| title_full_unstemmed | Single-molecule analysis of G protein-coupled receptor stoichiometry: Approaches and limitations |
| title_short | Single-molecule analysis of G protein-coupled receptor stoichiometry: Approaches and limitations |
| title_sort | single molecule analysis of g protein coupled receptor stoichiometry approaches and limitations |
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