Detrending: how to correct images for bleaching

Image series is affected by bleaching must be corrected by 'detrending' prior to the performance of quantitative analysis. There are several detrending methods (polynomial detrend, running average, exponential smoothing). We assess which methods are best, using examples from fluorescence f...

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Bibliographic Details
Main Authors: Nolan, R, Alvarez, L, Padilla-Parra, S
Format: Conference item
Published: Cell Press 2018