Detrending: how to correct images for bleaching
Image series is affected by bleaching must be corrected by 'detrending' prior to the performance of quantitative analysis. There are several detrending methods (polynomial detrend, running average, exponential smoothing). We assess which methods are best, using examples from fluorescence f...
| Main Authors: | , , |
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| Format: | Conference item |
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Cell Press
2018
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| _version_ | 1826284369320345600 |
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| author | Nolan, R Alvarez, L Padilla-Parra, S |
| author_facet | Nolan, R Alvarez, L Padilla-Parra, S |
| author_sort | Nolan, R |
| collection | OXFORD |
| description | Image series is affected by bleaching must be corrected by 'detrending' prior to the performance of quantitative analysis. There are several detrending methods (polynomial detrend, running average, exponential smoothing). We assess which methods are best, using examples from fluorescence fluctuation spectroscopy. We outline mistakes that are often made when detrending. We provide a free, user-friendly software for correctly detrending images. |
| first_indexed | 2024-03-07T01:12:48Z |
| format | Conference item |
| id | oxford-uuid:8d9d736c-0fcb-4dc1-aabc-cb6dca2d69fd |
| institution | University of Oxford |
| last_indexed | 2024-03-07T01:12:48Z |
| publishDate | 2018 |
| publisher | Cell Press |
| record_format | dspace |
| spelling | oxford-uuid:8d9d736c-0fcb-4dc1-aabc-cb6dca2d69fd2022-03-26T22:52:18ZDetrending: how to correct images for bleachingConference itemhttp://purl.org/coar/resource_type/c_c94fuuid:8d9d736c-0fcb-4dc1-aabc-cb6dca2d69fdSymplectic Elements at OxfordCell Press2018Nolan, RAlvarez, LPadilla-Parra, SImage series is affected by bleaching must be corrected by 'detrending' prior to the performance of quantitative analysis. There are several detrending methods (polynomial detrend, running average, exponential smoothing). We assess which methods are best, using examples from fluorescence fluctuation spectroscopy. We outline mistakes that are often made when detrending. We provide a free, user-friendly software for correctly detrending images. |
| spellingShingle | Nolan, R Alvarez, L Padilla-Parra, S Detrending: how to correct images for bleaching |
| title | Detrending: how to correct images for bleaching |
| title_full | Detrending: how to correct images for bleaching |
| title_fullStr | Detrending: how to correct images for bleaching |
| title_full_unstemmed | Detrending: how to correct images for bleaching |
| title_short | Detrending: how to correct images for bleaching |
| title_sort | detrending how to correct images for bleaching |
| work_keys_str_mv | AT nolanr detrendinghowtocorrectimagesforbleaching AT alvarezl detrendinghowtocorrectimagesforbleaching AT padillaparras detrendinghowtocorrectimagesforbleaching |