Efficient reverse click labeling of azide oligonucleotides with multiple alkynyl Cy-Dyes applied to the synthesis of HyBeacon probes for genetic analysis

Efficient reverse click labeling of azide oligonucleotides with multiple alkynyl Cy-Dyes applied to the synthesis of HyBeacon probes for genetic analysis

A convenient method of oligonucleotide labeling using click chemistry has been developed. A 2′-mesyloxyethyl ribothymidine phosphoramidite monomer was incorporated into DNA at several loci during solid phase oligonucleotide synthesis and converted to 2′-azidoethyl ribothymidine in high yield on the...

书目详细资料
Main Authors:	Gerowska, M, Hall, L, Richardson, J, Shelbourne, M, Brown, T
格式:	Journal article
语言:	English
出版:	2012

相似书籍

Six-colour HyBeacon probes for multiplex genetic analysis.
由: Richardson, J, et al.
出版: (2010)

HyBeacons: A new technology for genetic analysis
由: McDowell, D, et al.
出版: (2000)

HyBeacons®: A novel DNA probe chemistry for rapid genetic analysis
由: French, D, et al.
出版: (2006)

End-capped HyBeacon probes for the analysis of human genetic polymorphisms related to warfarin metabolism.
由: Ben Gaied, N, et al.
出版: (2010)

HyBeacon probes: a new tool for DNA sequence detection and allele discrimination.
由: French, D, et al.
出版: (2001)

Rapid typing of STRs in the human genome by HyBeacon melting.
由: Gale, N, et al.
出版: (2008)

Synthesis of HyBeacons and dual-labelled probes containing 2'-fluorescent groups for use in genetic analysis.
由: Dobson, N, et al.
出版: (2003)

Synthesis of HyBeacons and dual-labelled probes containing 2'-fluorescent groups for use in genetic analysis.
由: Dobson, N, et al.
出版: (2003)

PNA HyBeacons for analysis of human mutations related to statin-induced myopathy.
由: Gale, N, et al.
出版: (2011)

A highly fluorescent DNA toolkit: synthesis and properties of oligonucleotides containing new Cy3, Cy5 and Cy3B monomers.
由: Hall, L, et al.
出版: (2012)

Synthesis of alkyne- and azide-modified oligonucleotides and their cyclization by the CuAAC (click) reaction.
由: El-Sagheer, A, et al.
出版: (2008)

Synthesis of alkyne- and azide-modified oligonucleotides and their cyclization by the CuAAC (click) reaction.
由: El-Sagheer, A, et al.
出版: (2008)

Fast copper-free click DNA ligation by the ring-strain promoted alkyne-azide cycloaddition reaction.
由: Shelbourne, M, et al.
出版: (2011)

Azide and trans-cyclooctene dUTPs: incorporation into DNA probes and fluorescent click-labelling.
由: Ren, X, et al.
出版: (2015)

Microarrays: Indirect CyDye Labeling
由: Kristie Nybo
出版: (2009-09-01)

Fast, Cell-Compatible Click Chemistry with Copper-Chelating Azides for Biomolecular Labeling
由: Uttamapinant, Chayasith, et al.
出版: (2012)

Applications of Azide-Based Bioorthogonal Click Chemistry in Glycobiology
由: Xiu Zhang, et al.
出版: (2013-06-01)

A Genetically Encoded Picolyl Azide for Improved Live Cell Copper Click Labeling
由: Birthe Meineke, et al.
出版: (2021-11-01)

Alkyne-Azide “Click” Chemistry in Designing Nanocarriers for Applications in Biology
由: Pramod K. Avti, et al.
出版: (2013-08-01)

Copper-azide nanoparticle: a ‘catalyst-cum-reagent’ for the designing of 5-alkynyl 1,4-disubstituted triazoles
由: Debkumar Nandi, et al.
出版: (2020-10-01)

Fast and efficient DNA crosslinking and multiple orthogonal labelling by copper-free click chemistry.
由: Shelbourne, M, et al.
出版: (2012)

The application of click reactions in the synthesis of modified oligonucleotides
由: Qiu, J
出版: (2016)

Oligonucleotide Tagging for Copper-Free Click Conjugation
由: Alan Rowan, et al.
出版: (2013-06-01)

Sequence-dependence of Cy3 and Cy5 dyes in 3ʹ terminally-labeled single-stranded DNA
由: Tadija Kekić, et al.
出版: (2022-08-01)

Rapid and selective detection of fatty acylated proteins using ω-alkynyl-fatty acids and click chemistry[S]
由: Megan C. Yap, et al.
出版: (2010-06-01)

Oligonucleotide Labelling Using a Fluorogenic “Click” Reaction with a Hemicarboxonium Salt
由: Corinne Payrastre, et al.
出版: (2013-10-01)

Synthesis and Structure of <i>Nido</i>-Carboranyl Azide and Its “Click” Reactions
由: Anna A. Druzina, et al.
出版: (2021-01-01)

Overview of 1,5-Selective Click Reaction of Azides with Alkynes or Their Synthetic Equivalents
由: Yaqi Zhao, et al.
出版: (2023-02-01)

Dataset on characterization of hemin-azide derivative and DNA oligonucleotide-hemin conjugate
由: J. Kosman, et al.
出版: (2017-08-01)

Mass spectrometric analysis of maleimide CyDye labelled model peptides.
由: Wheeler, J, et al.
出版: (2003)

Protocol to identify S-acylated proteins in hippocampal neurons using ω-alkynyl fatty acid analogs and click chemistry
由: Charlotte A. Townsend, et al.
出版: (2024-06-01)

Exploiting azide-alkyne click chemistry in the synthesis, tracking and targeting of platinum anticancer complexes.
由: Farrer, NJ, et al.
出版: (2020)

Azide click chemistry on magnetotactic bacteria: A versatile technique to attach a cargo
由: Paul Eduardo David Soto Rodriguez, et al.
出版: (2023-04-01)

Modified Nucleosides, Nucleotides and Nucleic Acids via Click Azide-Alkyne Cycloaddition for Pharmacological Applications
由: Daniela Perrone, et al.
出版: (2021-05-01)

Layer-by-Layer Assembly of Microgel Colloidal Crystals via Photoinitiated Alkyne–Azide Click Reaction
由: Shuang Liang, et al.
出版: (2019-03-01)

Well-Defined Diimine Copper(I) Complexes as Catalysts in Click Azide-Alkyne Cycloaddition Reactions
由: Silvia Díez-González, et al.
出版: (2013-07-01)

Factors influencing hairpin oligonucleotide cyclization by the uncatalyzed alkyne-azide cycloaddition (AAC) reaction
由: El-Sagheer, A, et al.
出版: (2010)

Sub-Nanomolar Detection of Oligonucleotides Using Molecular Beacons Immobilized on Lightguiding Nanowires
由: Therese B. Johansson, et al.
出版: (2024-02-01)

Pharmacokinetics on a microscale: visualizing Cy5-labeled oligonucleotide release from poly(n-butylcyanoacrylate) nanocapsules in cells
由: Tomcin S, et al.
出版: (2014-11-01)

Arylethynyl- or Alkynyl-Linked Pyrimidine and 7‑Deazapurine 2′-Deoxyribonucleoside 3′-Phosphoramidites for Chemical Synthesis of Hypermodified Hydrophobic Oligonucleotides
由: Ivana Jestřábová, et al.
出版: (2023-10-01)