Phylometabonomic patterns of adaptation to high fat diet feeding in inbred mice.
Insulin resistance plays a central role in type 2 diabetes and obesity, which develop as a consequence of genetic and environmental factors. Dietary changes including high fat diet (HFD) feeding promotes insulin resistance in rodent models which present useful systems for studying interactions betwe...
| Hoofdauteurs: | , , , , , , , , , , , , |
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| Formaat: | Journal article |
| Taal: | English |
| Gepubliceerd in: |
Public Library of Science
2008
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| _version_ | 1826284727067213824 |
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| author | Fearnside, J Dumas, M Rothwell, A Wilder, S Cloarec, O Toye, A Blancher, C Holmes, E Tatoud, R Barton, R Scott, J Nicholson, J Gauguier, D |
| author_facet | Fearnside, J Dumas, M Rothwell, A Wilder, S Cloarec, O Toye, A Blancher, C Holmes, E Tatoud, R Barton, R Scott, J Nicholson, J Gauguier, D |
| author_sort | Fearnside, J |
| collection | OXFORD |
| description | Insulin resistance plays a central role in type 2 diabetes and obesity, which develop as a consequence of genetic and environmental factors. Dietary changes including high fat diet (HFD) feeding promotes insulin resistance in rodent models which present useful systems for studying interactions between genetic background and environmental influences contributing to disease susceptibility and progression. We applied a combination of classical physiological, biochemical and hormonal studies and plasma (1)H NMR spectroscopy-based metabonomics to characterize the phenotypic and metabotypic consequences of HFD (40%) feeding in inbred mouse strains (C57BL/6, 129S6, BALB/c, DBA/2, C3H) frequently used in genetic studies. We showed the wide range of phenotypic and metabonomic adaptations to HFD across the five strains and the increased nutrigenomic predisposition of 129S6 and C57BL/6 to insulin resistance and obesity relative to the other strains. In contrast mice of the BALB/c and DBA/2 strains showed relative resistance to HFD-induced glucose intolerance and obesity. Hierarchical metabonomic clustering derived from (1)H NMR spectral data of the strains provided a phylometabonomic classification of strain-specific metabolic features and differential responses to HFD which closely match SNP-based phylogenetic relationships between strains. Our results support the concept of genomic clustering of functionally related genes and provide important information for defining biological markers predicting spontaneous susceptibility to insulin resistance and pathological adaptations to fat feeding. |
| first_indexed | 2024-03-07T01:18:11Z |
| format | Journal article |
| id | oxford-uuid:8f6e5b67-d9a2-4ec9-b30c-f49cc20bee65 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T01:18:11Z |
| publishDate | 2008 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | oxford-uuid:8f6e5b67-d9a2-4ec9-b30c-f49cc20bee652022-03-26T23:04:10ZPhylometabonomic patterns of adaptation to high fat diet feeding in inbred mice.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:8f6e5b67-d9a2-4ec9-b30c-f49cc20bee65EnglishSymplectic Elements at OxfordPublic Library of Science2008Fearnside, JDumas, MRothwell, AWilder, SCloarec, OToye, ABlancher, CHolmes, ETatoud, RBarton, RScott, JNicholson, JGauguier, DInsulin resistance plays a central role in type 2 diabetes and obesity, which develop as a consequence of genetic and environmental factors. Dietary changes including high fat diet (HFD) feeding promotes insulin resistance in rodent models which present useful systems for studying interactions between genetic background and environmental influences contributing to disease susceptibility and progression. We applied a combination of classical physiological, biochemical and hormonal studies and plasma (1)H NMR spectroscopy-based metabonomics to characterize the phenotypic and metabotypic consequences of HFD (40%) feeding in inbred mouse strains (C57BL/6, 129S6, BALB/c, DBA/2, C3H) frequently used in genetic studies. We showed the wide range of phenotypic and metabonomic adaptations to HFD across the five strains and the increased nutrigenomic predisposition of 129S6 and C57BL/6 to insulin resistance and obesity relative to the other strains. In contrast mice of the BALB/c and DBA/2 strains showed relative resistance to HFD-induced glucose intolerance and obesity. Hierarchical metabonomic clustering derived from (1)H NMR spectral data of the strains provided a phylometabonomic classification of strain-specific metabolic features and differential responses to HFD which closely match SNP-based phylogenetic relationships between strains. Our results support the concept of genomic clustering of functionally related genes and provide important information for defining biological markers predicting spontaneous susceptibility to insulin resistance and pathological adaptations to fat feeding. |
| spellingShingle | Fearnside, J Dumas, M Rothwell, A Wilder, S Cloarec, O Toye, A Blancher, C Holmes, E Tatoud, R Barton, R Scott, J Nicholson, J Gauguier, D Phylometabonomic patterns of adaptation to high fat diet feeding in inbred mice. |
| title | Phylometabonomic patterns of adaptation to high fat diet feeding in inbred mice. |
| title_full | Phylometabonomic patterns of adaptation to high fat diet feeding in inbred mice. |
| title_fullStr | Phylometabonomic patterns of adaptation to high fat diet feeding in inbred mice. |
| title_full_unstemmed | Phylometabonomic patterns of adaptation to high fat diet feeding in inbred mice. |
| title_short | Phylometabonomic patterns of adaptation to high fat diet feeding in inbred mice. |
| title_sort | phylometabonomic patterns of adaptation to high fat diet feeding in inbred mice |
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