2'-Alkynylnucleotides: A Sequence- and Spin Label-Flexible Strategy for EPR Spectroscopy in DNA
Electron paramagnetic resonance (EPR) spectroscopy is a powerful method to elucidate molecular structure through the measurement of distances between conformationally well-defined spin labels. Here we report a sequence-flexible approach to the synthesis of double spin-labeled DNA duplexes, where 2′-...
Main Authors: | , , , , , , |
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Format: | Journal article |
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American Chemical Society
2016
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_version_ | 1797081867440095232 |
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author | Anderson, E El-Sagheer, A Porter, R Brown, T Haugland, M Peña, J Lovett, J |
author_facet | Anderson, E El-Sagheer, A Porter, R Brown, T Haugland, M Peña, J Lovett, J |
author_sort | Anderson, E |
collection | OXFORD |
description | Electron paramagnetic resonance (EPR) spectroscopy is a powerful method to elucidate molecular structure through the measurement of distances between conformationally well-defined spin labels. Here we report a sequence-flexible approach to the synthesis of double spin-labeled DNA duplexes, where 2′-alkynylnucleosides are incorporated at terminal and internal positions on complementary strands. Post-DNA synthesis copper-catalyzed azide–alkyne cycloaddition (CuAAC) reactions with a variety of spin labels enable the use of double electron–electron resonance experiments to measure a number of distances on the duplex, affording a high level of detailed structural information. |
first_indexed | 2024-03-07T01:20:07Z |
format | Journal article |
id | oxford-uuid:90051d24-0105-48b3-a77f-b6e39406366a |
institution | University of Oxford |
last_indexed | 2024-03-07T01:20:07Z |
publishDate | 2016 |
publisher | American Chemical Society |
record_format | dspace |
spelling | oxford-uuid:90051d24-0105-48b3-a77f-b6e39406366a2022-03-26T23:08:45Z2'-Alkynylnucleotides: A Sequence- and Spin Label-Flexible Strategy for EPR Spectroscopy in DNAJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:90051d24-0105-48b3-a77f-b6e39406366aSymplectic Elements at OxfordAmerican Chemical Society2016Anderson, EEl-Sagheer, APorter, RBrown, THaugland, MPeña, JLovett, JElectron paramagnetic resonance (EPR) spectroscopy is a powerful method to elucidate molecular structure through the measurement of distances between conformationally well-defined spin labels. Here we report a sequence-flexible approach to the synthesis of double spin-labeled DNA duplexes, where 2′-alkynylnucleosides are incorporated at terminal and internal positions on complementary strands. Post-DNA synthesis copper-catalyzed azide–alkyne cycloaddition (CuAAC) reactions with a variety of spin labels enable the use of double electron–electron resonance experiments to measure a number of distances on the duplex, affording a high level of detailed structural information. |
spellingShingle | Anderson, E El-Sagheer, A Porter, R Brown, T Haugland, M Peña, J Lovett, J 2'-Alkynylnucleotides: A Sequence- and Spin Label-Flexible Strategy for EPR Spectroscopy in DNA |
title | 2'-Alkynylnucleotides: A Sequence- and Spin Label-Flexible Strategy for EPR Spectroscopy in DNA |
title_full | 2'-Alkynylnucleotides: A Sequence- and Spin Label-Flexible Strategy for EPR Spectroscopy in DNA |
title_fullStr | 2'-Alkynylnucleotides: A Sequence- and Spin Label-Flexible Strategy for EPR Spectroscopy in DNA |
title_full_unstemmed | 2'-Alkynylnucleotides: A Sequence- and Spin Label-Flexible Strategy for EPR Spectroscopy in DNA |
title_short | 2'-Alkynylnucleotides: A Sequence- and Spin Label-Flexible Strategy for EPR Spectroscopy in DNA |
title_sort | 2 alkynylnucleotides a sequence and spin label flexible strategy for epr spectroscopy in dna |
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