Characterization of organic fluorophores for in vivo FRET studies based on electroporated molecules.

In vivo single-molecule fluorescence and Förster resonance energy transfer (FRET) techniques are excellent tools for studying spatial distribution, the nanoscale structure and conformational changes in living cells. We have recently introduced an electroporation-based method to internalize DNA and p...

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Bibliographic Details
Main Authors: Plochowietz, A, Crawford, R, Kapanidis, A
Format: Journal article
Language:English
Published: 2014