Characterization of organic fluorophores for in vivo FRET studies based on electroporated molecules.

Characterization of organic fluorophores for in vivo FRET studies based on electroporated molecules.

In vivo single-molecule fluorescence and Förster resonance energy transfer (FRET) techniques are excellent tools for studying spatial distribution, the nanoscale structure and conformational changes in living cells. We have recently introduced an electroporation-based method to internalize DNA and p...

Бүрэн тодорхойлолт

Номзүйн дэлгэрэнгүй
Үндсэн зохиолчид:	Plochowietz, A, Crawford, R, Kapanidis, A
Формат:	Journal article
Хэл сонгох:	English
Хэвлэсэн:	2014

Ижил төстэй зүйлс

Single-molecule FRET measurements in bacterial cells
-н: Aigrain, L, зэрэг
Хэвлэсэн: (2012)

Long-lived intracellular single-molecule fluorescence using electroporated molecules.
-н: Crawford, R, зэрэг
Хэвлэсэн: (2013)

Single-Molecule Fluorescence and FRET Measurements on Internalized Proteins in Living Bacteria
-н: Aigrain, L, зэрэг
Хэвлэсэн: (2013)

Single-molecule FRET studies in live bacteria
-н: Plochowietz, A
Хэвлэсэн: (2016)

Single-Molecule FRET in Living Bacteria
-н: Crawford, R, зэрэг
Хэвлэсэн: (2012)

Characterizing single-molecule FRET dynamics with probability distribution analysis.
-н: Santoso, Y, зэрэг
Хэвлэсэн: (2010)

Stable end-sealed DNA as robust nano-rulers for in vivo single-molecule fluorescence
-н: Plochowietz, A, зэрэг
Хэвлэсэн: (2016)

Quantification of Small Molecule–Protein Interactions using FRET between Tryptophan and the Pacific Blue Fluorophore
-н: Molly M. Lee, зэрэг
Хэвлэсэн: (2016-12-01)

Characterization of dark quencher chromophores as nonfluorescent acceptors for single-molecule FRET.
-н: Le Reste, L, зэрэг
Хэвлэсэн: (2012)

Optimized delivery of fluorescently labeled proteins in live bacteria using electroporation.
-н: Sustarsic, M, зэрэг
Хэвлэсэн: (2014)

Solution-based single-molecule FRET studies of K(+) channel gating in a lipid bilayer..
-н: Sadler, E, зэрэг
Хэвлэсэн: (2016)

Solution-based single-molecule FRET studies of K(+) channel gating in a lipid bilayer
-н: Sadler, E, зэрэг
Хэвлэсэн: (2016)

Single-molecule FRET analysis of protein-DNA complexes.
-н: Heilemann, M, зэрэг
Хэвлэсэн: (2009)

Internalization of Fluorescent Biomolecules for Long-Lived Single-Molecule Observation in Living Bacteria
-н: Crawford, R, зэрэг
Хэвлэсэн: (2013)

Bleaching-resistant,near-continuous single-molecule fluorescence and fret based on fluorogenic and transient DNA binding
-н: Kümmerlin, M, зэрэг
Хэвлэсэн: (2023)

Tethered fluorophore motion: studying large DNA conformational changes by single-fluorophore imaging.
-н: May, P, зэрэг
Хэвлэсэн: (2014)

Defining the limits of single-molecule FRET resolution in TIRF microscopy.
-н: Holden, S, зэрэг
Хэвлэсэн: (2010)

Improved temporal resolution and linked hidden Markov modeling for switchable single-molecule FRET.
-н: Uphoff, S, зэрэг
Хэвлэсэн: (2011)

Identifying molecular dynamics in single-molecule FRET experiments with burst variance analysis.
-н: Torella, J, зэрэг
Хэвлэсэн: (2011)

New FRET Methods for Studying Processing of Nucleic Acids by Protein Machines
-н: Kapanidis, A
Хэвлэсэн: (2014)

Three-Fluorophore FRET Enables the Analysis of Ternary Protein Association in Living Plant Cells
-н: Nina Glöckner, зэрэг
Хэвлэсэн: (2022-10-01)

Fluorophore-gold nanoparticle FRET/plasmonic lasers with the streptavidin-biotin complex as the acceptor–donor linkage
-н: J. A. Rivera, зэрэг
Хэвлэсэн: (2021-12-01)

Conformational transitions in DNA polymerase I revealed by single-molecule FRET.
-н: Santoso, Y, зэрэг
Хэвлэсэн: (2010)

Monitoring multiple distances within a single molecule using switchable FRET.
-н: Uphoff, S, зэрэг
Хэвлэсэн: (2010)

Single-molecule FRET reveals the pre-initiation and initiation conformations of influenza virus promoter RNA
-н: Robb, N, зэрэг
Хэвлэсэн: (2016)

Preparation of site-specifically fluorophore-labeled polyubiquitin chains for FRET studies of Cdc48 substrate processing
-н: Cameron Williams, зэрэг
Хэвлэсэн: (2023-12-01)

Single-molecule FRET reveals a corkscrew RNA structure for the polymerase-bound influenza virus promoter.
-н: Tomescu, A, зэрэг
Хэвлэсэн: (2014)

Six steps closer to FRET-driven structural biology.
-н: Craggs, T, зэрэг
Хэвлэсэн: (2012)

Robust evaluation of intermolecular FRET using a large Stokes shift fluorophore as a donor
-н: Carmen Santana-Calvo, зэрэг
Хэвлэсэн: (2018-10-01)

In vivo single-RNA tracking shows that most tRNA diffuses freely in live bacteria
-н: Plochowietz, A, зэрэг
Хэвлэсэн: (2016)

Single molecule imaging simulations with advanced fluorophore photophysics
-н: Dominique Bourgeois
Хэвлэсэн: (2023-01-01)

Confinement-free wide-field ratiometric tracking of single fluorescent molecules
-н: Gilboa, B, зэрэг
Хэвлэсэн: (2019)

Blinking characteristics of organic fluorophores for blink-based multiplexing
-н: Amelia G. Seabury, зэрэг
Хэвлэсэн: (2024-01-01)

Recent electroporation-based systems for intracellular molecule delivery
-н: Zaizai Dong, зэрэг
Хэвлэсэн: (2021-12-01)

Determination of FRET orientation factor between artificial fluorophore and photosynthetic light-harvesting 2 complex (LH2)
-н: Kazuhiro J. Fujimoto, зэрэг
Хэвлэсэн: (2022-09-01)

Real-time transcription initiation by E. coli RNA polymerase in vitro and in vivo
-н: Plochowietz, A, зэрэг
Хэвлэсэн: (2015)

Single-molecule fluorescence of internalised biomolecules in live bacteria
-н: Plochowietz, A
Хэвлэсэн: (2012)

Studying the organization of DNA repair by single-cell and single-molecule imaging.
-н: Uphoff, S, зэрэг
Хэвлэсэн: (2014)

In vivo quantitative FRET small animal imaging: Intensity versus lifetime-based FRET
-н: Jason T. Smith, зэрэг
Хэвлэсэн: (2023-06-01)

Switchable and Functional Fluorophores for Multidimensional Single-Molecule Localization Microscopy
-н: Yunshu Liu, зэрэг
Хэвлэсэн: (2023-06-01)