Bisulfite-free direct detection of 5-methylcytosine and 5-hydroxymethylcytosine at base resolution

Bisulfite-free direct detection of 5-methylcytosine and 5-hydroxymethylcytosine at base resolution

Bisulfite sequencing has been the gold standard for mapping DNA modifications including 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) for decades1,2,3,4. However, this harsh chemical treatment degrades the majority of the DNA and generates sequencing libraries with low complexity2,5,6. H...

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Hauptverfasser:	Liu, Y, Siejka-Zielinska, P, Velikova, G, Bi, Y, Yuan, F, Tomkova, M, Bai, C, Chen, L, Schuster-Boeckler, B, Song, C
Format:	Journal article
Veröffentlicht:	Springer Nature 2019

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