Temperature regulates NF-κB dynamics and function through timing of A20 transcription
NF-κB signaling plays a pivotal role in control of the inflammatory response. We investigated how the dynamics and function of NF-κB were affected by temperature within the mammalian physiological range (34 °C to 40 °C). An increase in temperature led to an increase in NF-κB nuclear/cytoplasmic osci...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Journal article |
| Published: |
National Academy of Sciences
2018
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| _version_ | 1797082668250169344 |
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| author | Harper, C Woodcock, D Lam, C Garcia-Albornoz, M Adamson, A Ashall, L Rowe, W Downton, P Schmidt, L West, S Spiller, D Rand, D White, M |
| author_facet | Harper, C Woodcock, D Lam, C Garcia-Albornoz, M Adamson, A Ashall, L Rowe, W Downton, P Schmidt, L West, S Spiller, D Rand, D White, M |
| author_sort | Harper, C |
| collection | OXFORD |
| description | NF-κB signaling plays a pivotal role in control of the inflammatory response. We investigated how the dynamics and function of NF-κB were affected by temperature within the mammalian physiological range (34 °C to 40 °C). An increase in temperature led to an increase in NF-κB nuclear/cytoplasmic oscillation frequency following Tumor Necrosis Factor alpha (TNFα) stimulation. Mathematical modeling suggested that this temperature sensitivity might be due to an A20-dependent mechanism, and A20 silencing removed the sensitivity to increased temperature. The timing of the early response of a key set of NF-κB target genes showed strong temperature dependence. The cytokine-induced expression of many (but not all) later genes was insensitive to temperature change (suggesting that they might be functionally temperature-compensated). Moreover, a set of temperature- and TNFα-regulated genes were implicated in NF-κB cross-talk with key cell-fate–controlling pathways. In conclusion, NF-κB dynamics and target gene expression are modulated by temperature and can accurately transmit multidimensional information to control inflammation. |
| first_indexed | 2024-03-07T01:31:01Z |
| format | Journal article |
| id | oxford-uuid:9398eba5-3cfa-46c1-b4f0-4b7f34a08fa8 |
| institution | University of Oxford |
| last_indexed | 2024-03-07T01:31:01Z |
| publishDate | 2018 |
| publisher | National Academy of Sciences |
| record_format | dspace |
| spelling | oxford-uuid:9398eba5-3cfa-46c1-b4f0-4b7f34a08fa82022-03-26T23:33:33ZTemperature regulates NF-κB dynamics and function through timing of A20 transcriptionJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:9398eba5-3cfa-46c1-b4f0-4b7f34a08fa8Symplectic Elements at OxfordNational Academy of Sciences2018Harper, CWoodcock, DLam, CGarcia-Albornoz, MAdamson, AAshall, LRowe, WDownton, PSchmidt, LWest, SSpiller, DRand, DWhite, MNF-κB signaling plays a pivotal role in control of the inflammatory response. We investigated how the dynamics and function of NF-κB were affected by temperature within the mammalian physiological range (34 °C to 40 °C). An increase in temperature led to an increase in NF-κB nuclear/cytoplasmic oscillation frequency following Tumor Necrosis Factor alpha (TNFα) stimulation. Mathematical modeling suggested that this temperature sensitivity might be due to an A20-dependent mechanism, and A20 silencing removed the sensitivity to increased temperature. The timing of the early response of a key set of NF-κB target genes showed strong temperature dependence. The cytokine-induced expression of many (but not all) later genes was insensitive to temperature change (suggesting that they might be functionally temperature-compensated). Moreover, a set of temperature- and TNFα-regulated genes were implicated in NF-κB cross-talk with key cell-fate–controlling pathways. In conclusion, NF-κB dynamics and target gene expression are modulated by temperature and can accurately transmit multidimensional information to control inflammation. |
| spellingShingle | Harper, C Woodcock, D Lam, C Garcia-Albornoz, M Adamson, A Ashall, L Rowe, W Downton, P Schmidt, L West, S Spiller, D Rand, D White, M Temperature regulates NF-κB dynamics and function through timing of A20 transcription |
| title | Temperature regulates NF-κB dynamics and function through timing of A20 transcription |
| title_full | Temperature regulates NF-κB dynamics and function through timing of A20 transcription |
| title_fullStr | Temperature regulates NF-κB dynamics and function through timing of A20 transcription |
| title_full_unstemmed | Temperature regulates NF-κB dynamics and function through timing of A20 transcription |
| title_short | Temperature regulates NF-κB dynamics and function through timing of A20 transcription |
| title_sort | temperature regulates nf κb dynamics and function through timing of a20 transcription |
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