Large-scale amplification, cloning and sequencing of near full-length HIV-1 subtype C genomes.

Full-length HIV-1 genome sequencing provides important data needed to address several vaccine design, molecular epidemiologic and pathogenesis questions. A protocol is presented for obtaining near full-length genomes (NFLGs) from subjects infected with HIV-1 subtype C. This protocol was used to ampl...

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Bibliographic Details
Main Authors: Rousseau, C, Birditt, B, McKay, A, Stoddard, J, Lee, T, McLaughlin, S, Moore, S, Shindo, N, Learn, G, Korber, B, Brander, C, Goulder, P, Kiepiela, P, Walker, B, Mullins, J
Format: Journal article
Language:English
Published: 2006