Long-term imaging of mouse embryos using adaptive harmonic generation microscopy.

We present a detailed description of an adaptive harmonic generation (HG) microscope and culture techniques that permit long-term, three-dimensional imaging of mouse embryos. HG signal from both pre- and postimplantation stage (0.5-5.5 day-old) mouse embryos are fully characterized. The second HG im...

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Bibliographic Details
Main Authors: Thayil, A, Watanabe, T, Jesacher, A, Wilson, T, Srinivas, S, Booth, M
Format: Journal article
Language:English
Published: 2011