Development and application of a fluoride-detection-based fluorescence assay for γ-butyrobetaine hydroxylase.
Fluoride assays for oxygenases: The 2-oxoglutarate-dependent oxygenase BBOX catalyses the final step in carnitine biosynthesis and is a medicinal chemistry target. We report that BBOX can hydroxylate fluorinated substrates analogues with subsequent release of a fluoride ion, thereby enabling an effi...
| Main Authors: | , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2012
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| _version_ | 1797083415557701632 |
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| author | Rydzik, A Leung, I Kochan, G Thalhammer, A Oppermann, U Claridge, T Schofield, C |
| author_facet | Rydzik, A Leung, I Kochan, G Thalhammer, A Oppermann, U Claridge, T Schofield, C |
| author_sort | Rydzik, A |
| collection | OXFORD |
| description | Fluoride assays for oxygenases: The 2-oxoglutarate-dependent oxygenase BBOX catalyses the final step in carnitine biosynthesis and is a medicinal chemistry target. We report that BBOX can hydroxylate fluorinated substrates analogues with subsequent release of a fluoride ion, thereby enabling an efficient fluorescence-based assay. |
| first_indexed | 2024-03-07T01:41:29Z |
| format | Journal article |
| id | oxford-uuid:970286b3-133f-4f93-b4b0-f9da49add64b |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T01:41:29Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | oxford-uuid:970286b3-133f-4f93-b4b0-f9da49add64b2022-03-26T23:56:44ZDevelopment and application of a fluoride-detection-based fluorescence assay for γ-butyrobetaine hydroxylase.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:970286b3-133f-4f93-b4b0-f9da49add64bEnglishSymplectic Elements at Oxford2012Rydzik, ALeung, IKochan, GThalhammer, AOppermann, UClaridge, TSchofield, CFluoride assays for oxygenases: The 2-oxoglutarate-dependent oxygenase BBOX catalyses the final step in carnitine biosynthesis and is a medicinal chemistry target. We report that BBOX can hydroxylate fluorinated substrates analogues with subsequent release of a fluoride ion, thereby enabling an efficient fluorescence-based assay. |
| spellingShingle | Rydzik, A Leung, I Kochan, G Thalhammer, A Oppermann, U Claridge, T Schofield, C Development and application of a fluoride-detection-based fluorescence assay for γ-butyrobetaine hydroxylase. |
| title | Development and application of a fluoride-detection-based fluorescence assay for γ-butyrobetaine hydroxylase. |
| title_full | Development and application of a fluoride-detection-based fluorescence assay for γ-butyrobetaine hydroxylase. |
| title_fullStr | Development and application of a fluoride-detection-based fluorescence assay for γ-butyrobetaine hydroxylase. |
| title_full_unstemmed | Development and application of a fluoride-detection-based fluorescence assay for γ-butyrobetaine hydroxylase. |
| title_short | Development and application of a fluoride-detection-based fluorescence assay for γ-butyrobetaine hydroxylase. |
| title_sort | development and application of a fluoride detection based fluorescence assay for γ butyrobetaine hydroxylase |
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