The coreceptor CD2 uses plasma membrane microdomains to transduce signals in T cells.
The interaction between a T cell and an antigen-presenting cell (APC) can trigger a signaling response that leads to T cell activation. Prior studies have shown that ligation of the T cell receptor (TCR) triggers a signaling cascade that proceeds through the coalescence of TCR and various signaling...
| Main Authors: | , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
2009
|
| _version_ | 1826286515629588480 |
|---|---|
| author | Kaizuka, Y Douglass, A Vardhana, S Dustin, M Vale, R |
| author_facet | Kaizuka, Y Douglass, A Vardhana, S Dustin, M Vale, R |
| author_sort | Kaizuka, Y |
| collection | OXFORD |
| description | The interaction between a T cell and an antigen-presenting cell (APC) can trigger a signaling response that leads to T cell activation. Prior studies have shown that ligation of the T cell receptor (TCR) triggers a signaling cascade that proceeds through the coalescence of TCR and various signaling molecules (e.g., the kinase Lck and adaptor protein LAT [linker for T cell activation]) into microdomains on the plasma membrane. In this study, we investigated another ligand-receptor interaction (CD58-CD2) that facilities T cell activation using a model system consisting of Jurkat T cells interacting with a planar lipid bilayer that mimics an APC. We show that the binding of CD58 to CD2, in the absence of TCR activation, also induces signaling through the actin-dependent coalescence of signaling molecules (including TCR-zeta chain, Lck, and LAT) into microdomains. When simultaneously activated, TCR and CD2 initially colocalize in small microdomains but then partition into separate zones; this spatial segregation may enable the two receptors to enhance signaling synergistically. Our results show that two structurally distinct receptors both induce a rapid spatial reorganization of molecules in the plasma membrane, suggesting a model for how local increases in the concentration of signaling molecules can trigger T cell signaling. |
| first_indexed | 2024-03-07T01:44:55Z |
| format | Journal article |
| id | oxford-uuid:9816cf8a-45dc-422e-ac2e-ea3b840c4284 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T01:44:55Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | oxford-uuid:9816cf8a-45dc-422e-ac2e-ea3b840c42842022-03-27T00:04:34ZThe coreceptor CD2 uses plasma membrane microdomains to transduce signals in T cells.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:9816cf8a-45dc-422e-ac2e-ea3b840c4284EnglishSymplectic Elements at Oxford2009Kaizuka, YDouglass, AVardhana, SDustin, MVale, RThe interaction between a T cell and an antigen-presenting cell (APC) can trigger a signaling response that leads to T cell activation. Prior studies have shown that ligation of the T cell receptor (TCR) triggers a signaling cascade that proceeds through the coalescence of TCR and various signaling molecules (e.g., the kinase Lck and adaptor protein LAT [linker for T cell activation]) into microdomains on the plasma membrane. In this study, we investigated another ligand-receptor interaction (CD58-CD2) that facilities T cell activation using a model system consisting of Jurkat T cells interacting with a planar lipid bilayer that mimics an APC. We show that the binding of CD58 to CD2, in the absence of TCR activation, also induces signaling through the actin-dependent coalescence of signaling molecules (including TCR-zeta chain, Lck, and LAT) into microdomains. When simultaneously activated, TCR and CD2 initially colocalize in small microdomains but then partition into separate zones; this spatial segregation may enable the two receptors to enhance signaling synergistically. Our results show that two structurally distinct receptors both induce a rapid spatial reorganization of molecules in the plasma membrane, suggesting a model for how local increases in the concentration of signaling molecules can trigger T cell signaling. |
| spellingShingle | Kaizuka, Y Douglass, A Vardhana, S Dustin, M Vale, R The coreceptor CD2 uses plasma membrane microdomains to transduce signals in T cells. |
| title | The coreceptor CD2 uses plasma membrane microdomains to transduce signals in T cells. |
| title_full | The coreceptor CD2 uses plasma membrane microdomains to transduce signals in T cells. |
| title_fullStr | The coreceptor CD2 uses plasma membrane microdomains to transduce signals in T cells. |
| title_full_unstemmed | The coreceptor CD2 uses plasma membrane microdomains to transduce signals in T cells. |
| title_short | The coreceptor CD2 uses plasma membrane microdomains to transduce signals in T cells. |
| title_sort | coreceptor cd2 uses plasma membrane microdomains to transduce signals in t cells |
| work_keys_str_mv | AT kaizukay thecoreceptorcd2usesplasmamembranemicrodomainstotransducesignalsintcells AT douglassa thecoreceptorcd2usesplasmamembranemicrodomainstotransducesignalsintcells AT vardhanas thecoreceptorcd2usesplasmamembranemicrodomainstotransducesignalsintcells AT dustinm thecoreceptorcd2usesplasmamembranemicrodomainstotransducesignalsintcells AT valer thecoreceptorcd2usesplasmamembranemicrodomainstotransducesignalsintcells AT kaizukay coreceptorcd2usesplasmamembranemicrodomainstotransducesignalsintcells AT douglassa coreceptorcd2usesplasmamembranemicrodomainstotransducesignalsintcells AT vardhanas coreceptorcd2usesplasmamembranemicrodomainstotransducesignalsintcells AT dustinm coreceptorcd2usesplasmamembranemicrodomainstotransducesignalsintcells AT valer coreceptorcd2usesplasmamembranemicrodomainstotransducesignalsintcells |