Quantitative FRET analysis by fast acquisition time domain FLIM at high spatial resolution in living cells.
Quantitative analysis in Förster resonance energy transfer (FRET) experiments in live cells for protein interaction studies is still a challenging issue. In a two-component system (FRET and no FRET donor species), fitting of fluorescence lifetime imaging microscopy (FLIM) data gives the fraction of...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2008
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