Single-Molecule Imaging of Electroporated Dye-Labelled CheY in Live E. coli

For the last two decades, the use of genetically fused Fluorescent Proteins has greatly contributed to the study of chemotactic signalling in E. coli including the activation of the response regulator protein CheY and its interaction with the flagellar motor. However, this approach suffers from a nu...

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Bibliographic Details
Main Authors: Berry, R, Di Paolo, D, Afanzar, O, Armitage, J
Format: Journal article
Published: Royal Society 2016