Enzyme-less DNA base identification by chemical stepping in a nanopore
The stepwise movement of a single biopolymer strand through a nanoscopic detector for the sequential identification of its building blocks offers a universal means for single-molecule sequencing. This principle has been implemented in portable sequencers that use enzymes to move DNA or RNA through h...
| Main Authors: | , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
American Chemical Society
2021
|
| _version_ | 1797107983119810560 |
|---|---|
| author | Qing, Y Bayley, H |
| author_facet | Qing, Y Bayley, H |
| author_sort | Qing, Y |
| collection | OXFORD |
| description | The stepwise movement of a single biopolymer strand through a nanoscopic detector for the sequential identification of its building blocks offers a universal means for single-molecule sequencing. This principle has been implemented in portable sequencers that use enzymes to move DNA or RNA through hundreds of individual nanopore detectors positioned in an array. Nevertheless, its application to the sequencing of other biopolymers, including polypeptides and polysaccharides, has not progressed because suitable enzymes are lacking. Recently, we devised a purely chemical means to move molecules processively in steps comparable to the repeat distances in biopolymers. Here, with this chemical approach, we demonstrate sequential nucleobase identification during DNA translocation through a nanopore. Further, the relative location of a guanine modification with a chemotherapeutic platinum derivative is pinpointed with single-base resolution. After further development, chemical translocation might replace stepping by enzymes for highly parallel single-molecule biopolymer sequencing. |
| first_indexed | 2024-03-07T07:21:33Z |
| format | Journal article |
| id | oxford-uuid:9adb2aae-d925-484b-affb-9345a842a54a |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T07:21:33Z |
| publishDate | 2021 |
| publisher | American Chemical Society |
| record_format | dspace |
| spelling | oxford-uuid:9adb2aae-d925-484b-affb-9345a842a54a2022-10-20T10:08:27ZEnzyme-less DNA base identification by chemical stepping in a nanoporeJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:9adb2aae-d925-484b-affb-9345a842a54aEnglishSymplectic ElementsAmerican Chemical Society2021Qing, YBayley, HThe stepwise movement of a single biopolymer strand through a nanoscopic detector for the sequential identification of its building blocks offers a universal means for single-molecule sequencing. This principle has been implemented in portable sequencers that use enzymes to move DNA or RNA through hundreds of individual nanopore detectors positioned in an array. Nevertheless, its application to the sequencing of other biopolymers, including polypeptides and polysaccharides, has not progressed because suitable enzymes are lacking. Recently, we devised a purely chemical means to move molecules processively in steps comparable to the repeat distances in biopolymers. Here, with this chemical approach, we demonstrate sequential nucleobase identification during DNA translocation through a nanopore. Further, the relative location of a guanine modification with a chemotherapeutic platinum derivative is pinpointed with single-base resolution. After further development, chemical translocation might replace stepping by enzymes for highly parallel single-molecule biopolymer sequencing. |
| spellingShingle | Qing, Y Bayley, H Enzyme-less DNA base identification by chemical stepping in a nanopore |
| title | Enzyme-less DNA base identification by chemical stepping in a nanopore |
| title_full | Enzyme-less DNA base identification by chemical stepping in a nanopore |
| title_fullStr | Enzyme-less DNA base identification by chemical stepping in a nanopore |
| title_full_unstemmed | Enzyme-less DNA base identification by chemical stepping in a nanopore |
| title_short | Enzyme-less DNA base identification by chemical stepping in a nanopore |
| title_sort | enzyme less dna base identification by chemical stepping in a nanopore |
| work_keys_str_mv | AT qingy enzymelessdnabaseidentificationbychemicalsteppinginananopore AT bayleyh enzymelessdnabaseidentificationbychemicalsteppinginananopore |