Analysis of B cell repertoire dynamics following hepatitis B vaccination in humans, and enrichment of vaccine-specific antibody sequences
Generating a diverse B cell immunoglobulin repertoire is essential for protection against infection. The repertoire in humans can now be comprehensively measured by high-throughput sequencing. Using hepatitis B vaccination as a model, we determined how the total immunoglobulin sequence repertoire ch...
| Main Authors: | , , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
Elsevier
2015
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| _version_ | 1797084774130515968 |
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| author | Galson, J Trück, J Fowler, A Clutterbuck, E Münz, M Cerundolo, V Reinhard, C van der Most, R Pollard, A Lunter, G Kelly, D |
| author_facet | Galson, J Trück, J Fowler, A Clutterbuck, E Münz, M Cerundolo, V Reinhard, C van der Most, R Pollard, A Lunter, G Kelly, D |
| author_sort | Galson, J |
| collection | OXFORD |
| description | Generating a diverse B cell immunoglobulin repertoire is essential for protection against infection. The repertoire in humans can now be comprehensively measured by high-throughput sequencing. Using hepatitis B vaccination as a model, we determined how the total immunoglobulin sequence repertoire changes following antigen exposure in humans, and compared this to sequences from vaccine-specific sorted cells. Clonal sequence expansions were seen 7 days after vaccination, which correlated with vaccine-specific plasma cell numbers. These expansions caused an increase in mutation, and a decrease in diversity and complementarity-determining region 3 sequence length in the repertoire. We also saw an increase in sequence convergence between participants 14 and 21 days after vaccination, coinciding with an increase of vaccine-specific memory cells. These features allowed development of a model for in silico enrichment of vaccine-specific sequences from the total repertoire. Identifying antigen-specific sequences from total repertoire data could aid our understanding B cell driven immunity, and be used for disease diagnostics and vaccine evaluation. |
| first_indexed | 2024-03-07T01:59:44Z |
| format | Journal article |
| id | oxford-uuid:9cedb41f-4ec3-459e-92d3-a2524ed8f348 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T01:59:44Z |
| publishDate | 2015 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | oxford-uuid:9cedb41f-4ec3-459e-92d3-a2524ed8f3482022-03-27T00:39:36ZAnalysis of B cell repertoire dynamics following hepatitis B vaccination in humans, and enrichment of vaccine-specific antibody sequencesJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:9cedb41f-4ec3-459e-92d3-a2524ed8f348EnglishSymplectic Elements at OxfordElsevier2015Galson, JTrück, JFowler, AClutterbuck, EMünz, MCerundolo, VReinhard, Cvan der Most, RPollard, ALunter, GKelly, DGenerating a diverse B cell immunoglobulin repertoire is essential for protection against infection. The repertoire in humans can now be comprehensively measured by high-throughput sequencing. Using hepatitis B vaccination as a model, we determined how the total immunoglobulin sequence repertoire changes following antigen exposure in humans, and compared this to sequences from vaccine-specific sorted cells. Clonal sequence expansions were seen 7 days after vaccination, which correlated with vaccine-specific plasma cell numbers. These expansions caused an increase in mutation, and a decrease in diversity and complementarity-determining region 3 sequence length in the repertoire. We also saw an increase in sequence convergence between participants 14 and 21 days after vaccination, coinciding with an increase of vaccine-specific memory cells. These features allowed development of a model for in silico enrichment of vaccine-specific sequences from the total repertoire. Identifying antigen-specific sequences from total repertoire data could aid our understanding B cell driven immunity, and be used for disease diagnostics and vaccine evaluation. |
| spellingShingle | Galson, J Trück, J Fowler, A Clutterbuck, E Münz, M Cerundolo, V Reinhard, C van der Most, R Pollard, A Lunter, G Kelly, D Analysis of B cell repertoire dynamics following hepatitis B vaccination in humans, and enrichment of vaccine-specific antibody sequences |
| title | Analysis of B cell repertoire dynamics following hepatitis B vaccination in humans, and enrichment of vaccine-specific antibody sequences |
| title_full | Analysis of B cell repertoire dynamics following hepatitis B vaccination in humans, and enrichment of vaccine-specific antibody sequences |
| title_fullStr | Analysis of B cell repertoire dynamics following hepatitis B vaccination in humans, and enrichment of vaccine-specific antibody sequences |
| title_full_unstemmed | Analysis of B cell repertoire dynamics following hepatitis B vaccination in humans, and enrichment of vaccine-specific antibody sequences |
| title_short | Analysis of B cell repertoire dynamics following hepatitis B vaccination in humans, and enrichment of vaccine-specific antibody sequences |
| title_sort | analysis of b cell repertoire dynamics following hepatitis b vaccination in humans and enrichment of vaccine specific antibody sequences |
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