Three-color alternating-laser excitation of single molecules: monitoring multiple interactions and distances.

We introduce three-color alternating-laser excitation (3c-ALEX), a fluorescence resonance energy transfer (FRET) method that measures up to three intramolecular distances and complex interaction stoichiometries of single molecules in solution. This tool extends substantially the capabilities of two-...

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Bibliographic Details
Main Authors: Lee, N, Kapanidis, A, Koh, H, Korlann, Y, Ho, S, Kim, Y, Gassman, N, Kim, S, Weiss, S
Format: Journal article
Language:English
Published: 2007