Three-color alternating-laser excitation of single molecules: monitoring multiple interactions and distances.
We introduce three-color alternating-laser excitation (3c-ALEX), a fluorescence resonance energy transfer (FRET) method that measures up to three intramolecular distances and complex interaction stoichiometries of single molecules in solution. This tool extends substantially the capabilities of two-...
Main Authors: | , , , , , , , , |
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Format: | Journal article |
Language: | English |
Published: |
2007
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