Loss of major histocompatibility complex-encoded transporter associated with antigen presentation (TAP) in colorectal cancer.
Presentation of endogenous antigenic peptides to cytotoxic T lymphocytes (CTLs) is mediated by the major histocompatibility complex (MHC) class I molecules. These antigenic peptides derived from the cytoplasmic protein pool are transported by the recently described MHC-encoded transporters (TAP1 and...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
1994
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| _version_ | 1797084986422067200 |
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| author | Kaklamanis, L Townsend, A Doussis-Anagnostopoulou, I Mortensen, N Harris, A Gatter, K |
| author_facet | Kaklamanis, L Townsend, A Doussis-Anagnostopoulou, I Mortensen, N Harris, A Gatter, K |
| author_sort | Kaklamanis, L |
| collection | OXFORD |
| description | Presentation of endogenous antigenic peptides to cytotoxic T lymphocytes (CTLs) is mediated by the major histocompatibility complex (MHC) class I molecules. These antigenic peptides derived from the cytoplasmic protein pool are transported by the recently described MHC-encoded transporters (TAP1 and TAP2) into a pre-Golgi region where they take part in the assembly of MHC class I molecules. Using an affinity-purified polyclonal antibody (AK1.7) for TAP1, we analyzed 81 colorectal carcinomas, 32 adenomas, and the respective nonneoplastic mucosa. Loss of the transporter molecule (TAP1) was observed in 14% (11 of 81) of the carcinomas, either complete (7 of 11) or focal (4 of 11), whereas adenomas and normal mucosa were always positive. This study adds further information to the understanding of the mechanisms related to the defective presentation of the MHC class I molecules by tumor cells. |
| first_indexed | 2024-03-07T02:02:48Z |
| format | Journal article |
| id | oxford-uuid:9df143d8-b47a-493e-bfa0-9a1cafef2eb4 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T02:02:48Z |
| publishDate | 1994 |
| record_format | dspace |
| spelling | oxford-uuid:9df143d8-b47a-493e-bfa0-9a1cafef2eb42022-03-27T00:46:40ZLoss of major histocompatibility complex-encoded transporter associated with antigen presentation (TAP) in colorectal cancer.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:9df143d8-b47a-493e-bfa0-9a1cafef2eb4EnglishSymplectic Elements at Oxford1994Kaklamanis, LTownsend, ADoussis-Anagnostopoulou, IMortensen, NHarris, AGatter, KPresentation of endogenous antigenic peptides to cytotoxic T lymphocytes (CTLs) is mediated by the major histocompatibility complex (MHC) class I molecules. These antigenic peptides derived from the cytoplasmic protein pool are transported by the recently described MHC-encoded transporters (TAP1 and TAP2) into a pre-Golgi region where they take part in the assembly of MHC class I molecules. Using an affinity-purified polyclonal antibody (AK1.7) for TAP1, we analyzed 81 colorectal carcinomas, 32 adenomas, and the respective nonneoplastic mucosa. Loss of the transporter molecule (TAP1) was observed in 14% (11 of 81) of the carcinomas, either complete (7 of 11) or focal (4 of 11), whereas adenomas and normal mucosa were always positive. This study adds further information to the understanding of the mechanisms related to the defective presentation of the MHC class I molecules by tumor cells. |
| spellingShingle | Kaklamanis, L Townsend, A Doussis-Anagnostopoulou, I Mortensen, N Harris, A Gatter, K Loss of major histocompatibility complex-encoded transporter associated with antigen presentation (TAP) in colorectal cancer. |
| title | Loss of major histocompatibility complex-encoded transporter associated with antigen presentation (TAP) in colorectal cancer. |
| title_full | Loss of major histocompatibility complex-encoded transporter associated with antigen presentation (TAP) in colorectal cancer. |
| title_fullStr | Loss of major histocompatibility complex-encoded transporter associated with antigen presentation (TAP) in colorectal cancer. |
| title_full_unstemmed | Loss of major histocompatibility complex-encoded transporter associated with antigen presentation (TAP) in colorectal cancer. |
| title_short | Loss of major histocompatibility complex-encoded transporter associated with antigen presentation (TAP) in colorectal cancer. |
| title_sort | loss of major histocompatibility complex encoded transporter associated with antigen presentation tap in colorectal cancer |
| work_keys_str_mv | AT kaklamanisl lossofmajorhistocompatibilitycomplexencodedtransporterassociatedwithantigenpresentationtapincolorectalcancer AT townsenda lossofmajorhistocompatibilitycomplexencodedtransporterassociatedwithantigenpresentationtapincolorectalcancer AT doussisanagnostopouloui lossofmajorhistocompatibilitycomplexencodedtransporterassociatedwithantigenpresentationtapincolorectalcancer AT mortensenn lossofmajorhistocompatibilitycomplexencodedtransporterassociatedwithantigenpresentationtapincolorectalcancer AT harrisa lossofmajorhistocompatibilitycomplexencodedtransporterassociatedwithantigenpresentationtapincolorectalcancer AT gatterk lossofmajorhistocompatibilitycomplexencodedtransporterassociatedwithantigenpresentationtapincolorectalcancer |