Pathways for Holliday junction processing during homologous recombination in Saccharomyces cerevisiae.
The Saccharomyces cerevisiae Rmi1 protein is a component of the highly conserved Sgs1-Top3-Rmi1 complex. Deletion of SGS1, TOP3, or RMI1 is synthetically lethal when combined with the loss of the Mus81-Mms4 or Slx1-Slx4 endonucleases, which have been implicated in Holliday junction (HJ) resolution....
| Main Authors: | , , , , |
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| Format: | Journal article |
| Language: | English |
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2011
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| _version_ | 1797085242180239360 |
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| author | Ashton, T Mankouri, H Heidenblut, A McHugh, P Hickson, I |
| author_facet | Ashton, T Mankouri, H Heidenblut, A McHugh, P Hickson, I |
| author_sort | Ashton, T |
| collection | OXFORD |
| description | The Saccharomyces cerevisiae Rmi1 protein is a component of the highly conserved Sgs1-Top3-Rmi1 complex. Deletion of SGS1, TOP3, or RMI1 is synthetically lethal when combined with the loss of the Mus81-Mms4 or Slx1-Slx4 endonucleases, which have been implicated in Holliday junction (HJ) resolution. To investigate the causes of this synthetic lethality, we isolated a temperature-sensitive mutant of the RMI1 strain, referred to as the rmi1-1 mutant. At the restrictive temperature, this mutant phenocopies an rmi1Δ strain but behaves like the wild type at the permissive temperature. Following a transient exposure to methyl methanesulfonate, rmi1-1 mutants accumulate unprocessed homologous recombination repair (HRR) intermediates. These intermediates are slowly resolved at the restrictive temperature, revealing a redundant resolution activity when Rmi1 is impaired. This resolution depends on Mus81-Mms4 but not on either Slx1-Slx4 or another HJ resolvase, Yen1. Similar results were also observed when Top3 function was impaired. We propose that the Sgs1-Top3-Rmi1 complex constitutes the main pathway for the processing of HJ-containing HRR intermediates but that Mus81-Mms4 can also resolve these intermediates. |
| first_indexed | 2024-03-07T02:06:11Z |
| format | Journal article |
| id | oxford-uuid:9f12f3a3-dc09-45bd-8994-056cc772272c |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T02:06:11Z |
| publishDate | 2011 |
| record_format | dspace |
| spelling | oxford-uuid:9f12f3a3-dc09-45bd-8994-056cc772272c2022-03-27T00:54:38ZPathways for Holliday junction processing during homologous recombination in Saccharomyces cerevisiae.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:9f12f3a3-dc09-45bd-8994-056cc772272cEnglishSymplectic Elements at Oxford2011Ashton, TMankouri, HHeidenblut, AMcHugh, PHickson, IThe Saccharomyces cerevisiae Rmi1 protein is a component of the highly conserved Sgs1-Top3-Rmi1 complex. Deletion of SGS1, TOP3, or RMI1 is synthetically lethal when combined with the loss of the Mus81-Mms4 or Slx1-Slx4 endonucleases, which have been implicated in Holliday junction (HJ) resolution. To investigate the causes of this synthetic lethality, we isolated a temperature-sensitive mutant of the RMI1 strain, referred to as the rmi1-1 mutant. At the restrictive temperature, this mutant phenocopies an rmi1Δ strain but behaves like the wild type at the permissive temperature. Following a transient exposure to methyl methanesulfonate, rmi1-1 mutants accumulate unprocessed homologous recombination repair (HRR) intermediates. These intermediates are slowly resolved at the restrictive temperature, revealing a redundant resolution activity when Rmi1 is impaired. This resolution depends on Mus81-Mms4 but not on either Slx1-Slx4 or another HJ resolvase, Yen1. Similar results were also observed when Top3 function was impaired. We propose that the Sgs1-Top3-Rmi1 complex constitutes the main pathway for the processing of HJ-containing HRR intermediates but that Mus81-Mms4 can also resolve these intermediates. |
| spellingShingle | Ashton, T Mankouri, H Heidenblut, A McHugh, P Hickson, I Pathways for Holliday junction processing during homologous recombination in Saccharomyces cerevisiae. |
| title | Pathways for Holliday junction processing during homologous recombination in Saccharomyces cerevisiae. |
| title_full | Pathways for Holliday junction processing during homologous recombination in Saccharomyces cerevisiae. |
| title_fullStr | Pathways for Holliday junction processing during homologous recombination in Saccharomyces cerevisiae. |
| title_full_unstemmed | Pathways for Holliday junction processing during homologous recombination in Saccharomyces cerevisiae. |
| title_short | Pathways for Holliday junction processing during homologous recombination in Saccharomyces cerevisiae. |
| title_sort | pathways for holliday junction processing during homologous recombination in saccharomyces cerevisiae |
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