Mapping of HLA genes using pulsed-field gradient electrophoresis.
The technique of pulsed-field gradient electrophoresis (PFGE) allows the determination of gene linkage relationships since DNA fragments up to 2 Mb can be separated. PFGE was employed to study linkage of class I, II and III genes belonging to the human major histocompatibility (HLA) complex. The res...
| Huvudupphovsmän: | , , , |
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| Materialtyp: | Journal article |
| Språk: | English |
| Publicerad: |
1986
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| _version_ | 1826287942611501056 |
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| author | Ragoussis, J van der Bliek, A Trowsdale, J Ziegler, A |
| author_facet | Ragoussis, J van der Bliek, A Trowsdale, J Ziegler, A |
| author_sort | Ragoussis, J |
| collection | OXFORD |
| description | The technique of pulsed-field gradient electrophoresis (PFGE) allows the determination of gene linkage relationships since DNA fragments up to 2 Mb can be separated. PFGE was employed to study linkage of class I, II and III genes belonging to the human major histocompatibility (HLA) complex. The results establish that the class II DO beta and DZ alpha genes are linked with the DP subregion, centromeric to the DQ/DX-DR-C4 chromosomal segment, and allow us to estimate the minimal length of the entire HLA complex. |
| first_indexed | 2024-03-07T02:06:15Z |
| format | Journal article |
| id | oxford-uuid:9f176659-54da-4759-b565-d5a469a9d4e0 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T02:06:15Z |
| publishDate | 1986 |
| record_format | dspace |
| spelling | oxford-uuid:9f176659-54da-4759-b565-d5a469a9d4e02022-03-27T00:54:55ZMapping of HLA genes using pulsed-field gradient electrophoresis.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:9f176659-54da-4759-b565-d5a469a9d4e0EnglishSymplectic Elements at Oxford1986Ragoussis, Jvan der Bliek, ATrowsdale, JZiegler, AThe technique of pulsed-field gradient electrophoresis (PFGE) allows the determination of gene linkage relationships since DNA fragments up to 2 Mb can be separated. PFGE was employed to study linkage of class I, II and III genes belonging to the human major histocompatibility (HLA) complex. The results establish that the class II DO beta and DZ alpha genes are linked with the DP subregion, centromeric to the DQ/DX-DR-C4 chromosomal segment, and allow us to estimate the minimal length of the entire HLA complex. |
| spellingShingle | Ragoussis, J van der Bliek, A Trowsdale, J Ziegler, A Mapping of HLA genes using pulsed-field gradient electrophoresis. |
| title | Mapping of HLA genes using pulsed-field gradient electrophoresis. |
| title_full | Mapping of HLA genes using pulsed-field gradient electrophoresis. |
| title_fullStr | Mapping of HLA genes using pulsed-field gradient electrophoresis. |
| title_full_unstemmed | Mapping of HLA genes using pulsed-field gradient electrophoresis. |
| title_short | Mapping of HLA genes using pulsed-field gradient electrophoresis. |
| title_sort | mapping of hla genes using pulsed field gradient electrophoresis |
| work_keys_str_mv | AT ragoussisj mappingofhlagenesusingpulsedfieldgradientelectrophoresis AT vanderblieka mappingofhlagenesusingpulsedfieldgradientelectrophoresis AT trowsdalej mappingofhlagenesusingpulsedfieldgradientelectrophoresis AT zieglera mappingofhlagenesusingpulsedfieldgradientelectrophoresis |