Optimized delivery of fluorescently labeled proteins in live bacteria using electroporation.

Optimized delivery of fluorescently labeled proteins in live bacteria using electroporation.

Studying the structure and dynamics of proteins in live cells is essential to understanding their physiological activities and mechanisms, and to validating in vitro characterization. Improvements in labeling and imaging technologies are starting to allow such in vivo studies; however, a number of t...

書目詳細資料
Main Authors:	Sustarsic, M, Plochowietz, A, Aigrain, L, Yuzenkova, Y, Zenkin, N, Kapanidis, A
格式:	Journal article
語言:	English
出版:	2014

相似書籍

Optimized Internalization of Fluorescently Labeled Biomolecules into Live Bacteria
由: Sustarsic, M, et al.
出版: (2014)

Single-Molecule Fluorescence and FRET Measurements on Internalized Proteins in Living Bacteria
由: Aigrain, L, et al.
出版: (2013)

Long-lived intracellular single-molecule fluorescence using electroporated molecules.
由: Crawford, R, et al.
出版: (2013)

Internalization of Fluorescent Biomolecules for Long-Lived Single-Molecule Observation in Living Bacteria
由: Crawford, R, et al.
出版: (2013)

Characterization of organic fluorophores for in vivo FRET studies based on electroporated molecules.
由: Plochowietz, A, et al.
出版: (2014)

Single-molecule fluorescence of internalised biomolecules in live bacteria
由: Plochowietz, A
出版: (2012)

Imaging of single dye-labeled chemotaxis proteins in live bacteria using electroporation
由: Di Paolo, D, et al.
出版: (2018)

In vivo single-RNA tracking shows that most tRNA diffuses freely in live bacteria
由: Plochowietz, A, et al.
出版: (2016)

Single-molecule imaging of electroporated chemotaxis proteins in live bacteria
由: Di Paolo, D
出版: (2015)

Single-molecule FRET studies in live bacteria
由: Plochowietz, A
出版: (2016)

Stable end-sealed DNA as robust nano-rulers for in vivo single-molecule fluorescence
由: Plochowietz, A, et al.
出版: (2016)

Site-specific incorporation of fluorescent probes into protein: hexahistidine-tag-mediated fluorescent labeling with (Ni(2+):nitrilotriacetic Acid (n)-fluorochrome conjugates.
由: Kapanidis, A, et al.
出版: (2001)

Single-molecule FRET measurements in bacterial cells
由: Aigrain, L, et al.
出版: (2012)

Stable transformation of fluorescent proteins into Nosema bombycis by electroporation
由: Zhanqi Dong, et al.
出版: (2022-04-01)

Electroporation of Labeled Antibodies to Visualize Endogenous Proteins and Posttranslational Modifications in Living Metazoan Cell Types
由: Sascha Conic, et al.
出版: (2018-11-01)

Aberrant topologies of bacterial membrane proteins revealed by high sensitivity fluorescence labelling
由: Hickman, SJ, et al.
出版: (2023)

Confinement-free wide-field ratiometric tracking of single fluorescent molecules
由: Gilboa, B, et al.
出版: (2019)

Single-peptide DNA-dependent RNA polymerase homologous to multi-subunit RNA polymerase
由: David Forrest, et al.
出版: (2017-06-01)

Single-molecule imaging of electroporated dye-labelled CheY in live E. coli
由: Berry, R, et al.
出版: (2016)

Single-molecule imaging of electroporated dye-labelled CheY in live Escherichia coli
由: Di Paolo, D, et al.
出版: (2016)

Single-Molecule Imaging of Electroporated Dye-Labelled CheY in Live E. coli
由: Berry, R, et al.
出版: (2016)

Single-Molecule FRET in Living Bacteria
由: Crawford, R, et al.
出版: (2012)

Single-molecule and super-resolution imaging of transcription in living bacteria
由: Stracy, M, et al.
出版: (2017)

Reversible Electroporation–Mediated Liposomal Doxorubicin Delivery to Tumors Can Be Monitored With Zr-Labeled Reporter Nanoparticles
由: Govindarajan Srimathveeravalli PhD, et al.
出版: (2018-02-01)

Minimal genetically encoded tags for fluorescent protein labeling in living neurons
由: Aleksandra Arsić, et al.
出版: (2022-01-01)

Enhanced fluorescent imaging of proteins in live yeast cells using fluorescently labeled scFv
由: Ioannis Tsirkas, et al.
出版: (2023-06-01)

Rapid single-cell electroporation for labeling organotypic cultures
由: Steinmeyer, Joseph D. (Joseph Daly)
出版: (2010)

Electroporation of tissue and cells for drug delivery applications
由: Prausnitz, Mark R
出版: (2006)

A Review on Electroporation-Based Intracellular Delivery
由: Junfeng Shi, et al.
出版: (2018-11-01)

Well Plate–Based Localized Electroporation Workflow for Rapid Optimization of Intracellular Delivery
由: Cesar Patino, et al.
出版: (2024-07-01)

Imaging living obligate anaerobic bacteria with bilin-binding fluorescent proteins
由: Hannah E. Chia, et al.
出版: (2020-09-01)

Choosing the right label for single-molecule tracking in live bacteria: side-by-side comparison of photoactivatable fluorescent protein and Halo tag dyes
由: Banaz, N, et al.
出版: (2018)

Transfer of Green Fluorescent Protein (GFP) Gene to Betta splendens Embryos by Transfection and Electroporation Methods
由: Eni Kusrini, et al.
出版: (2018-08-01)

Improved tracking and resolution of bacteria in holographic microscopy using dye and fluorescent protein labeling
由: Jay eNadeau, et al.
出版: (2016-04-01)

Labeling Proteins Inside Living Cells Using External Fluorophores for Fluorescence Microscopy
由: Kai Wen Teng, et al.
出版: (2017-01-01)

Evaluation and Optimization of Protein Extraction From E. coli by Electroporation
由: Saša Haberl Meglič, et al.
出版: (2020-09-01)

Inactivation of antibiotic-resistant bacteria Escherichia coli by electroporation
由: Saša Haberl Meglič, et al.
出版: (2024-01-01)

Fluorescently Labelled Tau Protein
由: Saurabh Awasthi, et al.
出版: (2023-12-01)

In vivo electroporation mediated gene delivery to the beating heart.
由: Erick L Ayuni, et al.
出版: (2010-01-01)

Substrate conformational dynamics facilitate structure-specific recognition of gapped DNA by DNA polymerase
由: Craggs, T, et al.
出版: (2019)