Activation of the non-canonical inflammasome in mouse and human cells
The non-canonical inflammasome is a signaling platform that allows for the detection of cytoplasmic lipopolysaccharides (LPS) in immune and non-immune cells. Upon detection of LPS, this inflammasome activates the signaling proteases caspase-4 and -5 (in humans) and caspase-11 (in mice). Inflammatory...
| Main Authors: | , , |
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| Format: | Book section |
| Language: | English |
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Humana Press
2022
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| _version_ | 1797110755943776256 |
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| author | Bezbradica, JS Coll, RC Boucher, D |
| author2 | Abdul-Sater, AA |
| author_facet | Abdul-Sater, AA Bezbradica, JS Coll, RC Boucher, D |
| author_sort | Bezbradica, JS |
| collection | OXFORD |
| description | The non-canonical inflammasome is a signaling platform that allows for the detection of cytoplasmic lipopolysaccharides (LPS) in immune and non-immune cells. Upon detection of LPS, this inflammasome activates the signaling proteases caspase-4 and -5 (in humans) and caspase-11 (in mice). Inflammatory caspases activation leads to caspase self-processing and the cleavage of the pore-forming protein Gasdermin D (GSDMD). GSDMD N-terminal fragments oligomerize and form pores at the plasma membranes, leading to an inflammatory form of cell death called pyroptosis. Here, we describe a simple method to activate the non-canonical inflammasome in myeloid and epithelial cells and to measure its activity using cell death assay and immunoblotting. |
| first_indexed | 2024-03-07T07:59:19Z |
| format | Book section |
| id | oxford-uuid:a0305074-e42c-481d-8adf-f3f579c16c43 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T07:59:19Z |
| publishDate | 2022 |
| publisher | Humana Press |
| record_format | dspace |
| spelling | oxford-uuid:a0305074-e42c-481d-8adf-f3f579c16c432023-09-07T09:32:46ZActivation of the non-canonical inflammasome in mouse and human cellsBook sectionhttp://purl.org/coar/resource_type/c_3248uuid:a0305074-e42c-481d-8adf-f3f579c16c43EnglishSymplectic ElementsHumana Press2022Bezbradica, JSColl, RCBoucher, DAbdul-Sater, AAThe non-canonical inflammasome is a signaling platform that allows for the detection of cytoplasmic lipopolysaccharides (LPS) in immune and non-immune cells. Upon detection of LPS, this inflammasome activates the signaling proteases caspase-4 and -5 (in humans) and caspase-11 (in mice). Inflammatory caspases activation leads to caspase self-processing and the cleavage of the pore-forming protein Gasdermin D (GSDMD). GSDMD N-terminal fragments oligomerize and form pores at the plasma membranes, leading to an inflammatory form of cell death called pyroptosis. Here, we describe a simple method to activate the non-canonical inflammasome in myeloid and epithelial cells and to measure its activity using cell death assay and immunoblotting. |
| spellingShingle | Bezbradica, JS Coll, RC Boucher, D Activation of the non-canonical inflammasome in mouse and human cells |
| title | Activation of the non-canonical inflammasome in mouse and human cells |
| title_full | Activation of the non-canonical inflammasome in mouse and human cells |
| title_fullStr | Activation of the non-canonical inflammasome in mouse and human cells |
| title_full_unstemmed | Activation of the non-canonical inflammasome in mouse and human cells |
| title_short | Activation of the non-canonical inflammasome in mouse and human cells |
| title_sort | activation of the non canonical inflammasome in mouse and human cells |
| work_keys_str_mv | AT bezbradicajs activationofthenoncanonicalinflammasomeinmouseandhumancells AT collrc activationofthenoncanonicalinflammasomeinmouseandhumancells AT boucherd activationofthenoncanonicalinflammasomeinmouseandhumancells |