A triazole linkage that mimics the DNA phosphodiester group in living systems
We describe the development of a chemical process based on the CuAAC reaction (click chemistry) to ligate DNA strands and produce an unnatural triazole backbone linkage. The chemical reaction is templated by a complementary DNA splint which accelerates the reaction and provides the required specific...
| Main Authors: | , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
Cambridge University Press
2015
|
| _version_ | 1826288269953859584 |
|---|---|
| author | El-Sagheer, A Brown, T |
| author_facet | El-Sagheer, A Brown, T |
| author_sort | El-Sagheer, A |
| collection | OXFORD |
| description | We describe the development of a chemical process based on the CuAAC reaction (click chemistry) to ligate DNA strands and produce an unnatural triazole backbone linkage. The chemical reaction is templated by a complementary DNA splint which accelerates the reaction and provides the required specificity. The resultant 1,4-triazole linkage is read through by DNA and RNA polymerases and is biocompatible in bacterial and human cells. This work has implications for the synthesis of chemically modified genes and other large modified DNA and RNA constructs. |
| first_indexed | 2024-03-07T02:11:10Z |
| format | Journal article |
| id | oxford-uuid:a0b1ffdb-f5bb-4731-935f-53244bc6e887 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T02:11:10Z |
| publishDate | 2015 |
| publisher | Cambridge University Press |
| record_format | dspace |
| spelling | oxford-uuid:a0b1ffdb-f5bb-4731-935f-53244bc6e8872022-03-27T02:07:20ZA triazole linkage that mimics the DNA phosphodiester group in living systemsJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:a0b1ffdb-f5bb-4731-935f-53244bc6e887EnglishSymplectic Elements at OxfordCambridge University Press2015El-Sagheer, ABrown, TWe describe the development of a chemical process based on the CuAAC reaction (click chemistry) to ligate DNA strands and produce an unnatural triazole backbone linkage. The chemical reaction is templated by a complementary DNA splint which accelerates the reaction and provides the required specificity. The resultant 1,4-triazole linkage is read through by DNA and RNA polymerases and is biocompatible in bacterial and human cells. This work has implications for the synthesis of chemically modified genes and other large modified DNA and RNA constructs. |
| spellingShingle | El-Sagheer, A Brown, T A triazole linkage that mimics the DNA phosphodiester group in living systems |
| title | A triazole linkage that mimics the DNA phosphodiester group in living systems |
| title_full | A triazole linkage that mimics the DNA phosphodiester group in living systems |
| title_fullStr | A triazole linkage that mimics the DNA phosphodiester group in living systems |
| title_full_unstemmed | A triazole linkage that mimics the DNA phosphodiester group in living systems |
| title_short | A triazole linkage that mimics the DNA phosphodiester group in living systems |
| title_sort | triazole linkage that mimics the dna phosphodiester group in living systems |
| work_keys_str_mv | AT elsagheera atriazolelinkagethatmimicsthednaphosphodiestergroupinlivingsystems AT brownt atriazolelinkagethatmimicsthednaphosphodiestergroupinlivingsystems AT elsagheera triazolelinkagethatmimicsthednaphosphodiestergroupinlivingsystems AT brownt triazolelinkagethatmimicsthednaphosphodiestergroupinlivingsystems |