Microcarriers for high-pressure freezing and cryosectioning of adherent cells.
A method is described employing microcarrier spheres of cross-linked dextran for obtaining ultra- and semithin vitreous sections from high-pressure frozen anchorage-dependent (mammalian) cells. Avoiding trypsination or scraping cells off from the culture surface, the presented approach allows for cr...
| Main Authors: | , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2008
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