Terminally truncated isopenicillin N synthase generates a dithioester product: evidence for a thioaldehyde intermediate during catalysis and a new mode of reaction for non-heme iron oxidases

Isopenicillin N synthase (IPNS) catalyses the oxidation of a tripeptide, L-δ-(α-aminoadipoyl)-L-cysteinyl-D-valine (ACV), to isopenicillin N (IPN), the first-formed β-lactam in penicillin biosynthesis. IPNS catalysis is dependent upon an iron(II) cofactor and oxygen as co-substrate. In the absence o...

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Bibliographic Details
Main Authors: McNeill, LA, Brown, TJN, Sami, M, Clifton, IJ, Burzlaff, NI, Claridge, TDW, Adlington, RM, Baldwin, JE, Rutledge, PJ, Schofield, CJ
Format: Journal article
Published: Wiley 2017