SpySwitch enables pH- or heat-responsive capture and release for plug-and-display nanoassembly
Proteins can be empowered via SpyTag for anchoring and nanoassembly, through covalent bonding to SpyCatcher partners. Here we generate a switchable version of SpyCatcher, allowing gentle purification of SpyTagged proteins. We introduce numerous histidines adjacent to SpyTag’s binding site, giving mo...
| Autores principales: | , , , , , |
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| Formato: | Journal article |
| Lenguaje: | English |
| Publicado: |
Springer Nature
2022
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