The arthritis-associated HLA-B*27:05 allele forms more cell surface B27 dimer and free heavy chain ligands for KIR3DL2 than HLA-B*27:09.
OBJECTIVES: HLA-B*27:05 is associated with AS whereas HLA-B*27:09 is not associated. We hypothesized that different interactions with KIR immune receptors could contribute to the difference in disease association between HLA-B*27:05 and HLAB*27:09. Thus, the objective of this study was to compare th...
| Κύριοι συγγραφείς: | , , , , , , , , , , , , , , , , , , , , , , , |
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| Μορφή: | Journal article |
| Γλώσσα: | English |
| Έκδοση: |
Oxford University Press
2013
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| _version_ | 1826288903731019776 |
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| author | Cauli, A Shaw, J Giles, J Hatano, H Rysnik, O Payeli, S McHugh, K Dessole, G Porru, G Desogus, E Fiedler, S Hölper, S Carette, A Blanco-Gelaz, M Vacca, A Piga, M Ibba, V Garau, P La Nasa, G López-Larrea, C Mathieu, A Renner, C Bowness, P Kollnberger, S |
| author_facet | Cauli, A Shaw, J Giles, J Hatano, H Rysnik, O Payeli, S McHugh, K Dessole, G Porru, G Desogus, E Fiedler, S Hölper, S Carette, A Blanco-Gelaz, M Vacca, A Piga, M Ibba, V Garau, P La Nasa, G López-Larrea, C Mathieu, A Renner, C Bowness, P Kollnberger, S |
| author_sort | Cauli, A |
| collection | OXFORD |
| description | OBJECTIVES: HLA-B*27:05 is associated with AS whereas HLA-B*27:09 is not associated. We hypothesized that different interactions with KIR immune receptors could contribute to the difference in disease association between HLA-B*27:05 and HLAB*27:09. Thus, the objective of this study was to compare the formation of β2m-free heavy chain (FHC) including B27 dimers (B272) by HLA-B*27:05 and HLA-B*27:09 and their binding to KIR immunoreceptors. METHODS: We studied the formation of HLA-B*27:05 and HLA-B*27:09 heterotrimers and FHC forms including dimers in vitro and in transfected cells. We investigated HLA-B*27:05 and HLA-B*27:09 binding to KIR3DL1, KIR3DL2 and LILRB2 by FACS staining with class I tetramers and by quantifying interactions with KIR3DL2CD3ε-reporter cells and KIR3DL2-expressing NK cells. We also measured KIR expression on peripheral blood NK and CD4 T cells from 18 HLA-B*27:05 AS patients, 8 HLA-B27 negative and 12 HLA-B*27:05+ and HLA-B*27:09+ healthy controls by FACS staining. RESULTS: HLA-B*27:09 formed less B27₂ and FHC than HLA-B*27:05. HLA-B*27:05-expressing cells stimulated KIR3DL2CD3ε-reporter T cells more effectively. Cells expressing HLA-B*27:05 promoted KIR3DL2+ NK cell survival more strongly than HLA-B*27:09. HLA-B*27:05 and HLA-B*27:09 dimer tetramers stained KIR3DL1, KIR3DL2 and LILRB2 equivalently. Increased proportions of NK and CD4 T cells expressed KIR3DL2 in HLA-B*27:05+ AS patients compared with HLA-B*27:05+, HLA-B*27:09+ and HLA-B27- healthy controls. CONCLUSION: Differences in the formation of FHC ligands for KIR3DL2 by HLA-B*27:05 and HLA-B*27:09 could contribute to the differential association of these alleles with AS. |
| first_indexed | 2024-03-07T02:20:46Z |
| format | Journal article |
| id | oxford-uuid:a3d3c09d-988c-49eb-a6cd-2fb33ec0266a |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T02:20:46Z |
| publishDate | 2013 |
| publisher | Oxford University Press |
| record_format | dspace |
| spelling | oxford-uuid:a3d3c09d-988c-49eb-a6cd-2fb33ec0266a2022-03-27T02:29:51ZThe arthritis-associated HLA-B*27:05 allele forms more cell surface B27 dimer and free heavy chain ligands for KIR3DL2 than HLA-B*27:09.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:a3d3c09d-988c-49eb-a6cd-2fb33ec0266aEnglishSymplectic Elements at OxfordOxford University Press2013Cauli, AShaw, JGiles, JHatano, HRysnik, OPayeli, SMcHugh, KDessole, GPorru, GDesogus, EFiedler, SHölper, SCarette, ABlanco-Gelaz, MVacca, APiga, MIbba, VGarau, PLa Nasa, GLópez-Larrea, CMathieu, ARenner, CBowness, PKollnberger, SOBJECTIVES: HLA-B*27:05 is associated with AS whereas HLA-B*27:09 is not associated. We hypothesized that different interactions with KIR immune receptors could contribute to the difference in disease association between HLA-B*27:05 and HLAB*27:09. Thus, the objective of this study was to compare the formation of β2m-free heavy chain (FHC) including B27 dimers (B272) by HLA-B*27:05 and HLA-B*27:09 and their binding to KIR immunoreceptors. METHODS: We studied the formation of HLA-B*27:05 and HLA-B*27:09 heterotrimers and FHC forms including dimers in vitro and in transfected cells. We investigated HLA-B*27:05 and HLA-B*27:09 binding to KIR3DL1, KIR3DL2 and LILRB2 by FACS staining with class I tetramers and by quantifying interactions with KIR3DL2CD3ε-reporter cells and KIR3DL2-expressing NK cells. We also measured KIR expression on peripheral blood NK and CD4 T cells from 18 HLA-B*27:05 AS patients, 8 HLA-B27 negative and 12 HLA-B*27:05+ and HLA-B*27:09+ healthy controls by FACS staining. RESULTS: HLA-B*27:09 formed less B27₂ and FHC than HLA-B*27:05. HLA-B*27:05-expressing cells stimulated KIR3DL2CD3ε-reporter T cells more effectively. Cells expressing HLA-B*27:05 promoted KIR3DL2+ NK cell survival more strongly than HLA-B*27:09. HLA-B*27:05 and HLA-B*27:09 dimer tetramers stained KIR3DL1, KIR3DL2 and LILRB2 equivalently. Increased proportions of NK and CD4 T cells expressed KIR3DL2 in HLA-B*27:05+ AS patients compared with HLA-B*27:05+, HLA-B*27:09+ and HLA-B27- healthy controls. CONCLUSION: Differences in the formation of FHC ligands for KIR3DL2 by HLA-B*27:05 and HLA-B*27:09 could contribute to the differential association of these alleles with AS. |
| spellingShingle | Cauli, A Shaw, J Giles, J Hatano, H Rysnik, O Payeli, S McHugh, K Dessole, G Porru, G Desogus, E Fiedler, S Hölper, S Carette, A Blanco-Gelaz, M Vacca, A Piga, M Ibba, V Garau, P La Nasa, G López-Larrea, C Mathieu, A Renner, C Bowness, P Kollnberger, S The arthritis-associated HLA-B*27:05 allele forms more cell surface B27 dimer and free heavy chain ligands for KIR3DL2 than HLA-B*27:09. |
| title | The arthritis-associated HLA-B*27:05 allele forms more cell surface B27 dimer and free heavy chain ligands for KIR3DL2 than HLA-B*27:09. |
| title_full | The arthritis-associated HLA-B*27:05 allele forms more cell surface B27 dimer and free heavy chain ligands for KIR3DL2 than HLA-B*27:09. |
| title_fullStr | The arthritis-associated HLA-B*27:05 allele forms more cell surface B27 dimer and free heavy chain ligands for KIR3DL2 than HLA-B*27:09. |
| title_full_unstemmed | The arthritis-associated HLA-B*27:05 allele forms more cell surface B27 dimer and free heavy chain ligands for KIR3DL2 than HLA-B*27:09. |
| title_short | The arthritis-associated HLA-B*27:05 allele forms more cell surface B27 dimer and free heavy chain ligands for KIR3DL2 than HLA-B*27:09. |
| title_sort | arthritis associated hla b 27 05 allele forms more cell surface b27 dimer and free heavy chain ligands for kir3dl2 than hla b 27 09 |
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