Empty MHC class I molecules come out in the cold.
Major histocompatibility complex (MHC) class I molecules present antigen by transporting peptides from intracellularly degraded proteins to the cell surface for scrutiny by cytotoxic T cells. Recent work suggests that peptide binding may be required for efficient assembly and intracellular transport...
| Main Authors: | , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
1990
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| _version_ | 1826289182646992896 |
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| author | Ljunggren, H Stam, N Ohlén, C Neefjes, J Höglund, P Heemels, M Bastin, J Schumacher, T Townsend, A Kärre, K |
| author_facet | Ljunggren, H Stam, N Ohlén, C Neefjes, J Höglund, P Heemels, M Bastin, J Schumacher, T Townsend, A Kärre, K |
| author_sort | Ljunggren, H |
| collection | OXFORD |
| description | Major histocompatibility complex (MHC) class I molecules present antigen by transporting peptides from intracellularly degraded proteins to the cell surface for scrutiny by cytotoxic T cells. Recent work suggests that peptide binding may be required for efficient assembly and intracellular transport of MHC class I molecules, but it is not clear whether class I molecules can ever assemble in the absence of peptide. We report here that culture of the murine lymphoma mutant cell line RMA-S at reduced temperature (19-33 degrees C) promotes assembly, and results in a high level of cell surface expression of H-2/beta 2-microglobulin complexes that do not present endogenous antigens, and are labile at 37 degrees C. They can be stabilized at 37 degrees C by exposure to specific peptides known to interact with H-2Kb or Db. Our findings suggest that, in the absence of peptides, class I molecules can assemble but are unstable at body temperature. The induction of such molecules at reduced temperature opens new ways to analyse the nature of MHC class I peptide interactions at the cell surface. |
| first_indexed | 2024-03-07T02:25:00Z |
| format | Journal article |
| id | oxford-uuid:a54b2363-bd11-4272-b65f-9e8050958f55 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T02:25:00Z |
| publishDate | 1990 |
| record_format | dspace |
| spelling | oxford-uuid:a54b2363-bd11-4272-b65f-9e8050958f552022-03-27T02:39:27ZEmpty MHC class I molecules come out in the cold.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:a54b2363-bd11-4272-b65f-9e8050958f55EnglishSymplectic Elements at Oxford1990Ljunggren, HStam, NOhlén, CNeefjes, JHöglund, PHeemels, MBastin, JSchumacher, TTownsend, AKärre, KMajor histocompatibility complex (MHC) class I molecules present antigen by transporting peptides from intracellularly degraded proteins to the cell surface for scrutiny by cytotoxic T cells. Recent work suggests that peptide binding may be required for efficient assembly and intracellular transport of MHC class I molecules, but it is not clear whether class I molecules can ever assemble in the absence of peptide. We report here that culture of the murine lymphoma mutant cell line RMA-S at reduced temperature (19-33 degrees C) promotes assembly, and results in a high level of cell surface expression of H-2/beta 2-microglobulin complexes that do not present endogenous antigens, and are labile at 37 degrees C. They can be stabilized at 37 degrees C by exposure to specific peptides known to interact with H-2Kb or Db. Our findings suggest that, in the absence of peptides, class I molecules can assemble but are unstable at body temperature. The induction of such molecules at reduced temperature opens new ways to analyse the nature of MHC class I peptide interactions at the cell surface. |
| spellingShingle | Ljunggren, H Stam, N Ohlén, C Neefjes, J Höglund, P Heemels, M Bastin, J Schumacher, T Townsend, A Kärre, K Empty MHC class I molecules come out in the cold. |
| title | Empty MHC class I molecules come out in the cold. |
| title_full | Empty MHC class I molecules come out in the cold. |
| title_fullStr | Empty MHC class I molecules come out in the cold. |
| title_full_unstemmed | Empty MHC class I molecules come out in the cold. |
| title_short | Empty MHC class I molecules come out in the cold. |
| title_sort | empty mhc class i molecules come out in the cold |
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