TNFα signals through specialized factories where responsive coding and miRNA genes are transcribed.
Tumour necrosis factor alpha (TNFα) is a potent cytokine that signals through nuclear factor kappa B (NFκB) to activate a subset of human genes. It is usually assumed that this involves RNA polymerases transcribing responsive genes wherever they might be in the nucleus. Using primary human endotheli...
| Główni autorzy: | , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Journal article |
| Język: | English |
| Wydane: |
2012
|
| _version_ | 1826289463072915456 |
|---|---|
| author | Papantonis, A Kohro, T Baboo, S Larkin, J Deng, B Short, P Tsutsumi, S Taylor, S Kanki, Y Kobayashi, M Li, G Poh, H Ruan, X Aburatani, H Ruan, Y Kodama, T Wada, Y Cook, P |
| author_facet | Papantonis, A Kohro, T Baboo, S Larkin, J Deng, B Short, P Tsutsumi, S Taylor, S Kanki, Y Kobayashi, M Li, G Poh, H Ruan, X Aburatani, H Ruan, Y Kodama, T Wada, Y Cook, P |
| author_sort | Papantonis, A |
| collection | OXFORD |
| description | Tumour necrosis factor alpha (TNFα) is a potent cytokine that signals through nuclear factor kappa B (NFκB) to activate a subset of human genes. It is usually assumed that this involves RNA polymerases transcribing responsive genes wherever they might be in the nucleus. Using primary human endothelial cells, variants of chromosome conformation capture (including 4C and chromatin interaction analysis with paired-end tag sequencing), and fluorescence in situ hybridization to detect single nascent transcripts, we show that TNFα induces responsive genes to congregate in discrete 'NFκB factories'. Some factories further specialize in transcribing responsive genes encoding micro-RNAs that target downregulated mRNAs. We expect all signalling pathways to contain this extra leg, where responding genes are transcribed in analogous specialized factories. |
| first_indexed | 2024-03-07T02:29:15Z |
| format | Journal article |
| id | oxford-uuid:a6ae45ad-a212-47fd-9389-040967efd374 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T02:29:15Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | oxford-uuid:a6ae45ad-a212-47fd-9389-040967efd3742022-03-27T02:48:58ZTNFα signals through specialized factories where responsive coding and miRNA genes are transcribed.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:a6ae45ad-a212-47fd-9389-040967efd374EnglishSymplectic Elements at Oxford2012Papantonis, AKohro, TBaboo, SLarkin, JDeng, BShort, PTsutsumi, STaylor, SKanki, YKobayashi, MLi, GPoh, HRuan, XAburatani, HRuan, YKodama, TWada, YCook, PTumour necrosis factor alpha (TNFα) is a potent cytokine that signals through nuclear factor kappa B (NFκB) to activate a subset of human genes. It is usually assumed that this involves RNA polymerases transcribing responsive genes wherever they might be in the nucleus. Using primary human endothelial cells, variants of chromosome conformation capture (including 4C and chromatin interaction analysis with paired-end tag sequencing), and fluorescence in situ hybridization to detect single nascent transcripts, we show that TNFα induces responsive genes to congregate in discrete 'NFκB factories'. Some factories further specialize in transcribing responsive genes encoding micro-RNAs that target downregulated mRNAs. We expect all signalling pathways to contain this extra leg, where responding genes are transcribed in analogous specialized factories. |
| spellingShingle | Papantonis, A Kohro, T Baboo, S Larkin, J Deng, B Short, P Tsutsumi, S Taylor, S Kanki, Y Kobayashi, M Li, G Poh, H Ruan, X Aburatani, H Ruan, Y Kodama, T Wada, Y Cook, P TNFα signals through specialized factories where responsive coding and miRNA genes are transcribed. |
| title | TNFα signals through specialized factories where responsive coding and miRNA genes are transcribed. |
| title_full | TNFα signals through specialized factories where responsive coding and miRNA genes are transcribed. |
| title_fullStr | TNFα signals through specialized factories where responsive coding and miRNA genes are transcribed. |
| title_full_unstemmed | TNFα signals through specialized factories where responsive coding and miRNA genes are transcribed. |
| title_short | TNFα signals through specialized factories where responsive coding and miRNA genes are transcribed. |
| title_sort | tnfα signals through specialized factories where responsive coding and mirna genes are transcribed |
| work_keys_str_mv | AT papantonisa tnfasignalsthroughspecializedfactorieswhereresponsivecodingandmirnagenesaretranscribed AT kohrot tnfasignalsthroughspecializedfactorieswhereresponsivecodingandmirnagenesaretranscribed AT baboos tnfasignalsthroughspecializedfactorieswhereresponsivecodingandmirnagenesaretranscribed AT larkinj tnfasignalsthroughspecializedfactorieswhereresponsivecodingandmirnagenesaretranscribed AT dengb tnfasignalsthroughspecializedfactorieswhereresponsivecodingandmirnagenesaretranscribed AT shortp tnfasignalsthroughspecializedfactorieswhereresponsivecodingandmirnagenesaretranscribed AT tsutsumis tnfasignalsthroughspecializedfactorieswhereresponsivecodingandmirnagenesaretranscribed AT taylors tnfasignalsthroughspecializedfactorieswhereresponsivecodingandmirnagenesaretranscribed AT kankiy tnfasignalsthroughspecializedfactorieswhereresponsivecodingandmirnagenesaretranscribed AT kobayashim tnfasignalsthroughspecializedfactorieswhereresponsivecodingandmirnagenesaretranscribed AT lig tnfasignalsthroughspecializedfactorieswhereresponsivecodingandmirnagenesaretranscribed AT pohh tnfasignalsthroughspecializedfactorieswhereresponsivecodingandmirnagenesaretranscribed AT ruanx tnfasignalsthroughspecializedfactorieswhereresponsivecodingandmirnagenesaretranscribed AT aburatanih tnfasignalsthroughspecializedfactorieswhereresponsivecodingandmirnagenesaretranscribed AT ruany tnfasignalsthroughspecializedfactorieswhereresponsivecodingandmirnagenesaretranscribed AT kodamat tnfasignalsthroughspecializedfactorieswhereresponsivecodingandmirnagenesaretranscribed AT waday tnfasignalsthroughspecializedfactorieswhereresponsivecodingandmirnagenesaretranscribed AT cookp tnfasignalsthroughspecializedfactorieswhereresponsivecodingandmirnagenesaretranscribed |