Genes encoding the Ti beta subunit of the antigen/MHC receptor undergo rearrangement during intrathymic ontogeny prior to surface T3-Ti expression.
To obtain further information about the ontogeny of the T-cell antigen/MHC receptor, a Ti beta subunit cDNA probe and heteroantisera specific for the Ti alpha and Ti beta subunits were utilized to characterize human T-lineage cells. Analysis of thymic tumors and normal thymocytes at both the DNA and...
| Prif Awduron: | , , , , , , |
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| Fformat: | Journal article |
| Iaith: | English |
| Cyhoeddwyd: |
1984
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| Crynodeb: | To obtain further information about the ontogeny of the T-cell antigen/MHC receptor, a Ti beta subunit cDNA probe and heteroantisera specific for the Ti alpha and Ti beta subunits were utilized to characterize human T-lineage cells. Analysis of thymic tumors and normal thymocytes at both the DNA and protein levels demonstrates that Ti beta gene rearrangement is evident in stage II (T11+T6+T3-) and stage III (T11+T6-T3+), but not stage I (T11+T6-T3-) thymocytes. In contrast, surface expression of Ti alpha and Ti beta molecules is exclusively restricted to stage III thymocytes. Thus human T-lineage ontogeny is characterized by an orderly series of differentiation steps wherein Ti beta gene rearrangement precedes surface expression of the T3-Ti molecular complex. |
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