Methods for qPCR gene expression profiling applied to 1440 lymphoblastoid single cells

The stochastic nature of generating eukaryotic transcripts challenges conventional methods for obtaining and analyzing single-cell gene expression data. In order to address the inherent noise, detailed methods are described on how to collect data on multiple genes in a large number of single cells u...

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Detalhes bibliográficos
Principais autores: Livak, K, Wills, Q, Tipping, A, Datta, K, Mittal, R, Goldson, A, Sexton, D, Holmes, C
Formato: Journal article
Idioma:English
Publicado em: 2013