Structure determination of noncanonical RNA motifs guided by ¹H NMR chemical shifts.
Structured noncoding RNAs underlie fundamental cellular processes, but determining their three-dimensional structures remains challenging. We demonstrate that integrating ¹H NMR chemical shift data with Rosetta de novo modeling can be used to consistently determine high-resolution RNA structures. On...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2014
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| _version_ | 1797087586381987840 |
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| author | Sripakdeevong, P Cevec, M Chang, A Erat, M Ziegeler, M Zhao, Q Fox, G Gao, X Kennedy, S Kierzek, R Nikonowicz, E Schwalbe, H Sigel, R Turner, D Das, R |
| author_facet | Sripakdeevong, P Cevec, M Chang, A Erat, M Ziegeler, M Zhao, Q Fox, G Gao, X Kennedy, S Kierzek, R Nikonowicz, E Schwalbe, H Sigel, R Turner, D Das, R |
| author_sort | Sripakdeevong, P |
| collection | OXFORD |
| description | Structured noncoding RNAs underlie fundamental cellular processes, but determining their three-dimensional structures remains challenging. We demonstrate that integrating ¹H NMR chemical shift data with Rosetta de novo modeling can be used to consistently determine high-resolution RNA structures. On a benchmark set of 23 noncanonical RNA motifs, including 11 'blind' targets, chemical-shift Rosetta for RNA (CS-Rosetta-RNA) recovered experimental structures with high accuracy (0.6-2.0 Å all-heavy-atom r.m.s. deviation) in 18 cases. |
| first_indexed | 2024-03-07T02:37:45Z |
| format | Journal article |
| id | oxford-uuid:a95a6b31-65f9-4e0b-89b5-e7de3b8c349e |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T02:37:45Z |
| publishDate | 2014 |
| record_format | dspace |
| spelling | oxford-uuid:a95a6b31-65f9-4e0b-89b5-e7de3b8c349e2022-03-27T03:07:58ZStructure determination of noncanonical RNA motifs guided by ¹H NMR chemical shifts.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:a95a6b31-65f9-4e0b-89b5-e7de3b8c349eEnglishSymplectic Elements at Oxford2014Sripakdeevong, PCevec, MChang, AErat, MZiegeler, MZhao, QFox, GGao, XKennedy, SKierzek, RNikonowicz, ESchwalbe, HSigel, RTurner, DDas, RStructured noncoding RNAs underlie fundamental cellular processes, but determining their three-dimensional structures remains challenging. We demonstrate that integrating ¹H NMR chemical shift data with Rosetta de novo modeling can be used to consistently determine high-resolution RNA structures. On a benchmark set of 23 noncanonical RNA motifs, including 11 'blind' targets, chemical-shift Rosetta for RNA (CS-Rosetta-RNA) recovered experimental structures with high accuracy (0.6-2.0 Å all-heavy-atom r.m.s. deviation) in 18 cases. |
| spellingShingle | Sripakdeevong, P Cevec, M Chang, A Erat, M Ziegeler, M Zhao, Q Fox, G Gao, X Kennedy, S Kierzek, R Nikonowicz, E Schwalbe, H Sigel, R Turner, D Das, R Structure determination of noncanonical RNA motifs guided by ¹H NMR chemical shifts. |
| title | Structure determination of noncanonical RNA motifs guided by ¹H NMR chemical shifts. |
| title_full | Structure determination of noncanonical RNA motifs guided by ¹H NMR chemical shifts. |
| title_fullStr | Structure determination of noncanonical RNA motifs guided by ¹H NMR chemical shifts. |
| title_full_unstemmed | Structure determination of noncanonical RNA motifs guided by ¹H NMR chemical shifts. |
| title_short | Structure determination of noncanonical RNA motifs guided by ¹H NMR chemical shifts. |
| title_sort | structure determination of noncanonical rna motifs guided by ¹h nmr chemical shifts |
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